Hendricks J B, Wilkinson E J, Kubilis P, Drew P, Blaydes S M, Munakata S
Department of Pathology, University of Florida, Gainesville.
Int J Gynecol Pathol. 1994 Jul;13(3):205-10. doi: 10.1097/00004347-199407000-00003.
The proliferative activity of invasive squamous cell carcinoma of the vulva was examined using a Ki-67 equivalent monoclonal antibody (MIB1), which gives a strong immunoreaction in paraffin-embedded tissue. Quantitation of Ki-67 immunostaining was accomplished by image analysis. Ki-67 immunostaining revealed two general patterns of reactivity in vulvar tumors: (a) a diffuse distribution of Ki-67 positive nuclei within the tumor mass and (b) a localized distribution of Ki-67 positive nuclei staining predominantly basilar components of tumor aggregates. The distribution of localized and diffuse patterns did not differ significantly between various clinicopathologic categories (age, histologic type and grade, FIGO stage, and lymph node status). However, the survival times for patients with a diffuse Ki-67 labelling pattern tended to be shorter than those for patients with a localized pattern. Survival curves based on the median positive nuclear area (PNA) calculated by image analysis did not differ significantly. Thus, the pattern of Ki-67 immunostaining, rather than the percentage of PNA, may have prognostic significance in vulvar squamous cell carcinoma.
使用一种Ki-67等效单克隆抗体（MIB1）对外阴浸润性鳞状细胞癌的增殖活性进行检测，该抗体在石蜡包埋组织中呈现强免疫反应。通过图像分析对Ki-67免疫染色进行定量。Ki-67免疫染色在外阴肿瘤中显示出两种一般反应模式：（a）肿瘤块内Ki-67阳性细胞核的弥漫性分布，以及（b）Ki-67阳性细胞核的局灶性分布，主要染色肿瘤聚集体的基底成分。在不同临床病理类别（年龄、组织学类型和分级、国际妇产科联盟（FIGO）分期和淋巴结状态）之间，局灶性和弥漫性模式的分布没有显著差异。然而，Ki-67弥漫性标记模式患者的生存时间往往比局灶性模式患者的生存时间短。基于图像分析计算的中位阳性核面积（PNA）的生存曲线没有显著差异。因此，Ki-67免疫染色模式而非PNA百分比可能对外阴鳞状细胞癌具有预后意义。
