• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

酵母杀伤因子RNA的5'末端是三磷酸鸟苷-磷酸。

The 5' ends of yeast killer factor RNAs are pppGp.

作者信息

Bruenn J, Keitz B

出版信息

Nucleic Acids Res. 1976 Oct;3(10):2427-36. doi: 10.1093/nar/3.10.2427.

DOI:10.1093/nar/3.10.2427
PMID:792814
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC343101/
Abstract

The 5' nucleotides of the double-stranded RNAs of yeast killer factor have been isolated by digestion with pancreatic, T1 and T2 RNase followed by two-dimensional electrophoresis. They were identified by bacterial alkaline phosphatase and snake venom phosphodiesterase digestions. Both the larger double-stranded RNA (L, of 2.5 x 10(6) daltons) and the smaller double-stranded RNA (M, of 1.4 x 10(6) daltons) have the 5' end groups pppGp. These 5' ends are dissimilar to those of the double-stranded RNAs of animal viruses but may be characteristic of the 5' ends of the double-stranded RNAs of fungal viruses.

摘要

酵母杀伤因子双链RNA的5'核苷酸已通过用胰核糖核酸酶、T1核糖核酸酶和T2核糖核酸酶消化，随后进行二维电泳分离得到。通过细菌碱性磷酸酶和蛇毒磷酸二酯酶消化对其进行了鉴定。较大的双链RNA（L，2.5×10⁶道尔顿）和较小的双链RNA（M，1.4×10⁶道尔顿）都具有5'端基团pppGp。这些5'端与动物病毒双链RNA的5'端不同，但可能是真菌病毒双链RNA 5'端的特征。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47ee/343101/3710cd183ba3/nar00495-0040-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47ee/343101/1b8f8f330077/nar00495-0036-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47ee/343101/6b0490a2d40d/nar00495-0039-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47ee/343101/3710cd183ba3/nar00495-0040-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47ee/343101/1b8f8f330077/nar00495-0036-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47ee/343101/6b0490a2d40d/nar00495-0039-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47ee/343101/3710cd183ba3/nar00495-0040-a.jpg

相似文献

1
The 5' ends of yeast killer factor RNAs are pppGp.酵母杀伤因子RNA的5'末端是三磷酸鸟苷-磷酸。
Nucleic Acids Res. 1976 Oct;3(10):2427-36. doi: 10.1093/nar/3.10.2427.
2
Yeast killer mutants with altered double-stranded ribonucleic acid.双链核糖核酸发生改变的酵母杀伤突变体。
J Bacteriol. 1974 Feb;117(2):681-6. doi: 10.1128/jb.117.2.681-686.1974.
3
Yeast viral double-stranded RNAs have heterogeneous 3' termini.酵母病毒双链RNA具有异质性的3'末端。
Cell. 1980 Apr;19(4):923-33. doi: 10.1016/0092-8674(80)90084-7.
4
No homology between double-stranded RNA and nuclear DNA of yeast.酵母的双链RNA与核DNA之间不存在同源性。
J Virol. 1978 Dec;28(3):1002-5. doi: 10.1128/JVI.28.3.1002-1005.1978.
5
Yeast positive-stranded virus-like RNA replicons. 20 S and 23 S RNA terminal nucleotide sequences and 3' end secondary structures resemble those of RNA coliphages.酵母正链病毒样RNA复制子。20S和23S RNA的末端核苷酸序列以及3'端二级结构与RNA噬菌体的相似。
J Biol Chem. 1998 Aug 7;273(32):20363-71. doi: 10.1074/jbc.273.32.20363.
6
Double-stranded RNA of homothallic Saccharomycetes with different cytoplasmic determinants of antagonistic activity.具有不同细胞质拮抗活性决定因素的同宗配合酵母的双链RNA。
Mol Biol (Mosk). 1976 Jul-Aug;10(4):767-71.
7
Saccharomyces cerevisiae killer virus transcripts contain template-coded polyadenylate tracts.酿酒酵母杀伤病毒转录本含有模板编码的聚腺苷酸序列。
Mol Cell Biol. 1984 Jan;4(1):101-9. doi: 10.1128/mcb.4.1.101-109.1984.
8
Relatedness of the double-stranded RNAs present in yeast virus-like particles.酵母病毒样颗粒中双链RNA的相关性。
J Virol. 1978 Jun;26(3):762-72. doi: 10.1128/JVI.26.3.762-772.1978.
9
Virus-like particles and double stranded RNA from killer and non-killer strains of Saccharomyces cerevisiae.来自酿酒酵母杀伤型和非杀伤型菌株的病毒样颗粒和双链RNA。
Microbios. 1978;21(85-86):161-76.
10
[Structural organization of double-stranded RNA from killer yeasts Saccharomyces cerevisiae by the thermal denaturation method].[利用热变性法对酿酒酵母杀伤酵母双链RNA的结构组织进行研究]
Mol Biol (Mosk). 1985 Nov-Dec;19(6):1579-84.

引用本文的文献

1
Cellular production of a counterfeit viral protein confers immunity to infection by a related virus.细胞产生的一种伪造病毒蛋白赋予对相关病毒感染的免疫力。
PeerJ. 2018 Sep 28;6:e5679. doi: 10.7717/peerj.5679. eCollection 2018.
2
XRN1 Is a Species-Specific Virus Restriction Factor in Yeasts.XRN1是酵母中一种物种特异性的病毒限制因子。
PLoS Pathog. 2016 Oct 6;12(10):e1005890. doi: 10.1371/journal.ppat.1005890. eCollection 2016 Oct.
3
Virus-host co-evolution under a modified nuclear genetic code.在经过修正的核遗传密码下，病毒-宿主的共同进化。

本文引用的文献

1
Blocked and unblocked 5' termini in reovirus genome RNA.呼肠孤病毒基因组RNA中封闭和未封闭的5'末端
J Virol. 1975 May;15(5):1057-64. doi: 10.1128/JVI.15.5.1057-1064.1975.
2
Terminal sequences of bacteriophage RNAs.噬菌体RNA的末端序列。
Nature. 1968 Nov 9;220(5167):548-52. doi: 10.1038/220548a0.
3
Studies on the nature of the killer factor produced by Saccharomyces cerevisiae.酿酒酵母产生的杀伤因子的性质研究。
PeerJ. 2013 Mar 5;1:e50. doi: 10.7717/peerj.50. Print 2013.
4
Yeast double-stranded RNA virus L-A deliberately synthesizes RNA transcripts with 5'-diphosphate.酵母双链 RNA 病毒 L-A 特意合成具有 5′-二磷酸的 RNA 转录本。
J Biol Chem. 2010 Jul 23;285(30):22911-8. doi: 10.1074/jbc.M110.138982. Epub 2010 May 28.
5
Identification of functionally important amino acids of ribosomal protein L3 by saturation mutagenesis.通过饱和诱变鉴定核糖体蛋白L3的功能重要氨基酸
Mol Cell Biol. 2005 Dec;25(24):10863-74. doi: 10.1128/MCB.25.24.10863-10874.2005.
6
Inhibition of 5' to 3' mRNA degradation under stress conditions in Saccharomyces cerevisiae: from GCN4 to MET16.酿酒酵母在应激条件下5'至3'mRNA降解的抑制作用：从GCN4到MET16
RNA. 2004 Mar;10(3):458-68. doi: 10.1261/rna.5183804.
7
The ski7 antiviral protein is an EF1-alpha homolog that blocks expression of non-Poly(A) mRNA in Saccharomyces cerevisiae.Ski7抗病毒蛋白是一种EF1-α同源物，可阻断酿酒酵母中非聚腺苷酸（Non-Poly(A)）mRNA的表达。
J Virol. 1999 Apr;73(4):2893-900. doi: 10.1128/JVI.73.4.2893-2900.1999.
8
Ribosomal protein L3 mutants alter translational fidelity and promote rapid loss of the yeast killer virus.核糖体蛋白L3突变体改变翻译保真度并促进酵母杀伤病毒的快速丧失。
Mol Cell Biol. 1999 Jan;19(1):384-91. doi: 10.1128/MCB.19.1.384.
9
Posttranscriptional control of gene expression in yeast.酵母中基因表达的转录后调控
Microbiol Mol Biol Rev. 1998 Dec;62(4):1492-553. doi: 10.1128/MMBR.62.4.1492-1553.1998.
10
Ski6p is a homolog of RNA-processing enzymes that affects translation of non-poly(A) mRNAs and 60S ribosomal subunit biogenesis.Ski6p是RNA加工酶的同源物，它影响非聚腺苷酸化mRNA的翻译以及60S核糖体亚基的生物合成。
Mol Cell Biol. 1998 May;18(5):2688-96. doi: 10.1128/MCB.18.5.2688.
J Gen Microbiol. 1968 Apr;51(1):115-26. doi: 10.1099/00221287-51-1-115.
4
Asynchronous synthesis of the complementary strands of the reovirus genome.呼肠孤病毒基因组互补链的异步合成。
Proc Natl Acad Sci U S A. 1971 Feb;68(2):505-8. doi: 10.1073/pnas.68.2.505.
5
The 5'-terminus of bacteriophage R17 RNA: pppGp.
J Mol Biol. 1968 Jan 14;31(1):51-61. doi: 10.1016/0022-2836(68)90053-3.
6
Virus-like particles associated with the double-stranded RNA species found in killer and sensitive strains of the yeast Saccharomyces cerevisiae.与在酿酒酵母的杀伤型和敏感型菌株中发现的双链RNA种类相关的病毒样颗粒。
J Gen Virol. 1974 Mar;22(3):387-94. doi: 10.1099/0022-1317-22-3-387.
7
Yeast killer mutants with altered double-stranded ribonucleic acid.双链核糖核酸发生改变的酵母杀伤突变体。
J Bacteriol. 1974 Feb;117(2):681-6. doi: 10.1128/jb.117.2.681-686.1974.
8
Preliminary characterization of two species of dsRNA in yeast and their relationship to the "killer" character.酵母中两种双链RNA的初步特性及其与“杀伤”特性的关系。
Nature. 1973 Sep 14;245(5420):81-6. doi: 10.1038/245081b0.
9
Retention of cytoplasmic killer determinants in yeast cells after removal of mitochondrial DNA by ethidium bromide.经溴化乙锭去除线粒体DNA后酵母细胞中细胞质杀伤决定簇的保留
Mol Gen Genet. 1973 May 28;122(4):323-30. doi: 10.1007/BF00269432.
10
The nucleotide sequence of Saccharomyces cerevisiae 5.8 S ribosomal ribonucleic acid.酿酒酵母5.8S核糖体核糖核酸的核苷酸序列。
J Biol Chem. 1973 Jun 10;248(11):3860-75.