Nesterova G F, Soom Y O, Perevozchikov A P
Mol Biol (Mosk). 1976 Jul-Aug;10(4):767-71.
Double-stranded RNA of high molecular weight is found in the cytoplasm of the "killer" strain M-437-2. The RNA is homogeneous and contains no single strand poly(A) portions. The double-stranded RNA is associated with the cytoplasmic microsomes of strain M-437-2 and is contained in the fraction of the cell contents of this strain that was used to infect germinating spores of the sensitive S. cerevisiae 768(1)-15 with the cytoplasmic determinant [k]. The absence of this RNA in cells of the initial sensitive strain 768(1)-15, and its appearance in the "killer" derivative of this strain produced by infection with the determinant [k] by means of the above-mentioned fraction, indicate that the killer activity is due to the double-stranded RNA. The retention of the double-stranded RNA in the cytoplasm of derivatives of the newly-formed killer strain, containing the cytoplasmic determinants [k(ts)] and [n] suggests also that these determinants are a modification of determinant [k].
在“杀伤”菌株M - 437 - 2的细胞质中发现了高分子量的双链RNA。该RNA是均匀的，不包含单链聚（A）部分。双链RNA与菌株M - 437 - 2的细胞质微粒体相关联，并存在于该菌株细胞内容物的一部分中，该部分用于通过细胞质决定因子[k]感染敏感酿酒酵母768(1)-15的萌发孢子。初始敏感菌株768(1)-15的细胞中不存在这种RNA，而通过上述部分用决定因子[k]感染产生的该菌株的“杀伤”衍生物中出现这种RNA，这表明杀伤活性归因于双链RNA。新形成的杀伤菌株的衍生物的细胞质中保留双链RNA，这些衍生物含有细胞质决定因子[k(ts)]和[n]，这也表明这些决定因子是决定因子[k]的一种修饰。
