Carlini M, Garofalo A, Rinaldi G, D'Agnano I, D'Angelo C, Zupi G, Vecchione A, Santoro E
Second Department of Surgery, Regina Elena Cancer Institute, Rome, Italy.
Int Surg. 1994 Apr-Jun;79(2):114-9.
The DNA content of 59 adenocarcinomas of the stomach in patients who had undergone subtotal or total gastrectomy more than 5 years before was measured. The DNA measurements were done by flow cytometry performed on Propidium Iodide--stained cells disaggregated from paraffin-embedded tissues. Fifty-nine evaluable good quality histograms of DNA ploidy patterns were obtained. The Proliferative Index (PI) was determined in 35 cases. The remaining 24 cases didn't show a reliable reading histograms. Of the 59 tumors, 33 (56%) were diploid and 26 (44%) were aneuploid; 19 showed a high PI (> or = 3.8%) and 16 a lower one. A statistically significant difference was found between the two groups (diploid/aneuploid and low/high PI) compared to the prognostic values known as T, N and Stage. 65% of the T3-T4 cancers, 54% of the N1-N2 lesions and 58% of the stage III and IV were found to be aneuploid. 73.7% of the 19 tumors presenting high PI, showed an aneuploid pattern. A high PI was found in 71.4% of the T3-T4 tumors. 77.4% of patients of the diploid group (any stage) survived at 5 years against 36% of those presenting aneuploid patterns. Patients with PI > or = 3.8% showed a 42.1% 5-year survival rate. A 94.4% 5-year survival rate of diploid and early stage cancers was documented against a 33.5% of aneuploid and advanced stage cancers.
对5年多前接受过胃次全切除术或全胃切除术的患者的59例胃腺癌的DNA含量进行了测量。DNA测量通过流式细胞术进行，该技术是对从石蜡包埋组织中解离出来的碘化丙啶染色细胞进行检测。获得了59个可评估的高质量DNA倍体模式直方图。在35例病例中测定了增殖指数（PI）。其余24例未显示出可靠的直方图读数。在这59个肿瘤中，33个（56%）为二倍体，26个（44%）为非整倍体；19个显示高PI（≥3.8%），16个显示低PI。与已知的预后值T、N和分期相比，两组（二倍体/非整倍体和低/高PI）之间存在统计学上的显著差异。发现65%的T3 - T4期癌症、54%的N1 - N2期病变以及58%的III期和IV期病变为非整倍体。在19个呈现高PI的肿瘤中，73.7%显示非整倍体模式。在71.4%的T3 - T4期肿瘤中发现高PI。二倍体组（任何分期）77.4%的患者存活5年，而非整倍体模式患者的这一比例为36%。PI≥3.8%的患者5年生存率为42.1%。记录显示二倍体和早期癌症的5年生存率为94.4%，而非整倍体和晚期癌症的这一比例为33.5%。
