Omejc M, Repse S, Bracko M
University Medical Center, Department of Gastroenterologic Surgery, Ljubljana, Slovenia.
Acta Chir Hung. 1997;36(1-4):266-9.
Flow cytometric analysis of DNA ploidy was performed on an archival material obtained from 79 patients with gastric cancer who underwent a potentially curative (R0) stomach resection with D2 lymphadenectomy, and evaluated its relationship to conventional pathologic parameters, TNM stage and prognosis. No significant association between DNA aneuploidy and either patients' sex, depth of tumor infiltration (pT), lymph node involvement (pN), histological type according to Ming, macroscopic type according to Borrmann or tumor localization was found. However, the incidence of DNA aneuploidy was significantly lower in tumors of diffuse type according to Lauren, in tumors of signet ring cell or undifferentiated type according to WHO classification, in poorly differentiated/undifferentiated tumors and in patients younger than 50 years. We found no significant difference in survival between patients with DNA aneuploid tumors and those with DNA diploid tumors. Although the prognosis of patients with tumors of lower DNA index (DI < 1.2) tended to be better than for those of higher DNA index (DI > 1.2), the difference did not reach a statistically significant level (p = 0.09). TNM stage, depth of tumor infiltration (pT) and lymph node involvement (pN) were the only factors that significantly affected survival in univariate analysis and both pT and pN retained their independent prognostic significance in multivariate analysis.
对79例行D2淋巴结清扫的潜在根治性（R0）胃切除术的胃癌患者的存档材料进行了DNA倍体的流式细胞术分析，并评估了其与传统病理参数、TNM分期及预后的关系。未发现DNA非整倍体与患者性别、肿瘤浸润深度（pT）、淋巴结受累情况（pN）、Ming组织学类型、Borrmann大体类型或肿瘤定位之间存在显著关联。然而，根据Lauren分类为弥漫型的肿瘤、根据WHO分类为印戒细胞或未分化型的肿瘤、低分化/未分化肿瘤以及年龄小于50岁的患者中，DNA非整倍体的发生率显著较低。我们发现DNA非整倍体肿瘤患者与DNA二倍体肿瘤患者的生存率无显著差异。尽管DNA指数较低（DI < 1.2）的肿瘤患者的预后往往优于DNA指数较高（DI > 1.2）的患者，但差异未达到统计学显著水平（p = 0.09）。TNM分期、肿瘤浸润深度（pT）和淋巴结受累情况（pN）是单因素分析中显著影响生存的唯一因素，并且在多因素分析中pT和pN均保留了其独立的预后意义。
