Kang Y S, Ko J S, Hwang S M
Department of Oral Anatomy, College of Dentistry, Seoul National University, Korea.
J Anat. 1994 Jun;184 ( Pt 3)(Pt 3):607-14.
The ultrastructure of the reversal line during alveolar bone remodelling was investigated in the rat. Surface bone remodelling along the periosteum of the mandible was induced by the extraction of opposing maxillary molars. With transmission EM the reversal line was seen to be composed of a superficial electron-dense amorphous layer and a deep filamentous layer at 7 d after extraction. The reversal line exhibited strong alkaline phosphatase activity and contained acid mucopolysaccharide. Scanning EM of the surface of the line, exposed by sonication in distilled water, showed papillary structures, the surface of which appeared granular and exhibited a crystalline appearance. The tips of collagen fibrils of new bone were attached to the top of the papillae in the front area of bone formation. It is suggested that the reversal line is involved in the coupling of bone resorption and formation.
在大鼠中研究了牙槽骨重塑过程中反转线的超微结构。通过拔除对侧上颌磨牙诱导下颌骨骨膜表面的骨重塑。拔牙后7天，透射电镜观察到反转线由浅层电子致密无定形层和深层丝状层组成。反转线表现出强碱性磷酸酶活性并含有酸性粘多糖。通过在蒸馏水中超声处理暴露的反转线表面的扫描电镜显示乳头状结构，其表面呈颗粒状并呈现晶体外观。新骨胶原纤维的尖端附着在骨形成前部乳头的顶部。提示反转线参与骨吸收与形成的耦联。
