Chen Li-li, Tang Qi, Yan Jie
Department of Stomatology, the Second Affiliated Hospital, College of Medical Science, Zhejiang University, Hangzhou 310009, China.
Zhongguo Zhong Yao Za Zhi. 2004 Jun;29(6):549-53.
To establish a rat model of experimental alveolar bone resorption and understand the therapeutic effect of a traditional Chinese medical herb Drynaria fortunei (DFS) on the rats suffering from alveolar bone resorption.
A SD rat model with experimental alveolar bone resorption was established by using injection with E. coli Lipopolysaccharide (E-LPS) in local tissue of the animals. A DFS preparation (DFS aqueous-extract) was extracted with distilled water. The modeled rats were administrated by perfusion with DFS aqueous-extract at a dosage of 15 g crude drug/kg once a day for 10, 20 and 30 d, respectively. The effects of DFS aqueous extract on experimental alveolar bone resorption were estimated by detections of serum alkali phosphate (ALP) activity, Ca2+ and osteocalcin (OC) levels, and TRAP stained osteoclast count, bone mineral density (BMD) measurement and HE stained histopathological examination of the tooth-periodontal samples.
In comparison with the controls, BMD values of the alveolar bones at experimental tooth sites of the tested rats with DFS aqueous-extract for 10 d administration were obviously increased (P < 0.05). In the tooth-periodontal samples from the rats for 10 d administration, disappearance of the osteoclast, decrease of Howship's lacuna numbers and formation of new non-calcified bone-like matrix attached by osteoblasts in alveolar bone at the root furcation from most of the samples occurred. Similar examining results in the tooth-periodontal samples from the rats for 20 and 30 d administrations were obtained, respectively. However, no statistically significant differences of the serum ALP activity, Ca2+ and OC levels among the tested rats for 10-30 d treatment and the controls (P > 0.05) were found.
The DFS aqueous extract has exact therapeutic effect on rat experimental alveolar bone resorption through suppressing bone resorption and promoting bone regeneration. Serum ALP activity, Ca2+ and osteocalcin (OC) levels can not be used as the effective index for examining alveolar bone resorption and regeneration in animal models.
建立大鼠实验性牙槽骨吸收模型，了解中药骨碎补对牙槽骨吸收大鼠的治疗作用。
通过向动物局部组织注射大肠杆菌脂多糖（E-LPS）建立SD大鼠实验性牙槽骨吸收模型。用蒸馏水提取骨碎补制剂（骨碎补水提取物）。将造模大鼠分别以15 g生药/kg的剂量每日一次灌胃给予骨碎补水提取物，连续给药10、20和30天。通过检测血清碱性磷酸酶（ALP）活性、Ca2+和骨钙素（OC）水平、抗酒石酸酸性磷酸酶（TRAP）染色破骨细胞计数、骨密度（BMD）测量以及牙齿-牙周样本的苏木精-伊红（HE）染色组织病理学检查，评估骨碎补水提取物对实验性牙槽骨吸收的影响。
与对照组相比，给予骨碎补水提取物10天的受试大鼠实验牙位牙槽骨的BMD值明显升高（P < 0.05）。在给药10天的大鼠牙齿-牙周样本中，大部分样本的牙槽骨根分叉处破骨细胞消失、Howship陷窝数量减少，有成骨细胞附着的新的未钙化骨样基质形成。分别在给药20天和30天的大鼠牙齿-牙周样本中获得了类似的检查结果。然而，在给药10 - 30天的受试大鼠与对照组之间，血清ALP活性、Ca2+和OC水平无统计学显著差异（P > 0.05）。
骨碎补水提取物通过抑制骨吸收和促进骨再生对大鼠实验性牙槽骨吸收具有确切的治疗作用。血清ALP活性、Ca2+和骨钙素（OC）水平不能作为动物模型中牙槽骨吸收和再生的有效检测指标。
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