Raphael Y, Athey B D, Wang Y, Lee M K, Altschuler R A
Kresge Hearing Research Institute, University of Michigan, Ann Arbor 48109-0506.
Hear Res. 1994 Jun 1;76(1-2):173-87. doi: 10.1016/0378-5955(94)90098-1.
Laser scanning confocal microscopy was used to determine the distribution of actin, spectrin and tubulin in whole mounts of the organ of Corti of guinea pig, monkey, rat and chinchilla. Actin, spectrin and tubulin were localized in all cell types in the auditory epithelium. No specialized cytoskeletal organization of tubulin was detected in the cytoplasmic domain of hair cells. The only specialized organization of actin and spectrin in the cytoplasmic domain was the infra-cuticular network, found exclusively in apical guinea pig outer hair cells. In contrast, the lateral wall of inner and outer hair cells contained a homogeneous distribution of label specific for actin and spectrin. The label intensity was similar in the base and the apex of the cochlea. These results indicate that the distribution of spectrin and actin in the auditory epithelium is similar to that in other epithelial cells, suggesting that actin and spectrin participate in the formation of cellular shape and possibly in docking molecules to the membrane.
激光扫描共聚焦显微镜用于确定豚鼠、猴子、大鼠和毛丝鼠耳蜗柯蒂氏器整装标本中肌动蛋白、血影蛋白和微管蛋白的分布。肌动蛋白、血影蛋白和微管蛋白定位于听觉上皮的所有细胞类型中。在毛细胞的细胞质区域未检测到微管蛋白有特殊的细胞骨架组织。在细胞质区域，肌动蛋白和血影蛋白唯一的特殊组织是表皮下网络，仅在豚鼠顶部外毛细胞中发现。相反，内毛细胞和外毛细胞的侧壁含有肌动蛋白和血影蛋白特异性标记的均匀分布。耳蜗底部和顶部的标记强度相似。这些结果表明，血影蛋白和肌动蛋白在听觉上皮中的分布与其他上皮细胞中的分布相似，提示肌动蛋白和血影蛋白参与细胞形状的形成，并可能参与分子与膜的对接。
