Attanasio G, Spongr V P, Henderson D
Hearing Research Laboratory, State University of New York at Buffalo 14214, USA.
Hear Res. 1994 Dec;81(1-2):199-207. doi: 10.1016/0378-5955(94)90165-1.
The distribution of the two cytoskeletal proteins, filamentous actin (F-actin) and fodrin, was investigated along the organ of Corti of the chinchilla using laser scanning confocal fluorescence microscopy. High intensity labeling of F-actin was seen in outer and inner hair cells, including the stereocilia. High intensity staining was also seen for fodrin in outer and inner hair cells, but not in their stereocilia. Staining intensity of both proteins along the lateral cell wall of the outer hair cells appeared to be greater in the middle and basal cochlear turns than in the apical turn. Pillars and Deiters cells also exhibited high intensity labeling of F-actin. The lack of significant differences in the distribution of fodrin between outer and inner hair cells makes the role of this protein in the active processes still unclear. Comparison of the distribution of F-actin and fodrin in the chinchilla with those reported in the guinea pigs suggest possible species differences.
利用激光扫描共聚焦荧光显微镜，研究了两种细胞骨架蛋白，即丝状肌动蛋白（F-肌动蛋白）和血影蛋白，在灰鼠柯蒂氏器中的分布情况。在包括静纤毛在内的外毛细胞和内毛细胞中可见F-肌动蛋白的高强度标记。血影蛋白在外毛细胞和内毛细胞中也可见高强度染色，但在其静纤毛中未见。外毛细胞外侧细胞壁上这两种蛋白的染色强度在耳蜗中部和基部转弯处似乎比顶部转弯处更大。支柱细胞和Dieters细胞也表现出F-肌动蛋白的高强度标记。外毛细胞和内毛细胞之间血影蛋白分布缺乏显著差异，使得该蛋白在活跃过程中的作用仍不明确。将灰鼠中F-肌动蛋白和血影蛋白的分布与豚鼠中报道的分布进行比较，提示可能存在物种差异。
