Jaeger R G, Fex J, Kachar B
Section on Structural Cell Biology, National Institute on Deafness and Other Communication Disorders, NIH, Rockville, MD 20850.
Hear Res. 1994 Jun 15;77(1-2):207-15. doi: 10.1016/0378-5955(94)90268-2.
The actin matrix of the cuticular plate, which supports the sensory stereocilia bundle, is coupled to the axial cytoskeleton of the hair cell through a well defined microtubule columnar framework. A collection of axial microtubules in a columnar organization penetrate deep into the dense actin matrix of the cuticular plate. Each microtubule displays at the end a 300-500 nm long fuzzy cap that enmeshes with the actin matrix of the cuticular plate. The microtubule associated proteins MAP-1A and MAP-1B were localized by confocal immunofluorescence to the point of microtubule insertion in the cuticular plate. These proteins are likely components of the microtubule capping structure and may mediate the interaction of the microtubules with the actin matrix. The structural interaction of the microtubules with the cuticular plate provides important mechanical coupling of the transduction apparatus to the axial cytoskeleton of the hair cell.
支持感觉静纤毛束的角质板肌动蛋白基质，通过一个明确的微管柱状框架与毛细胞的轴向细胞骨架相连。一组呈柱状排列的轴向微管深入到角质板致密的肌动蛋白基质中。每个微管末端都有一个300 - 500纳米长的模糊帽，它与角质板的肌动蛋白基质相互交织。微管相关蛋白MAP - 1A和MAP - 1B通过共聚焦免疫荧光定位在角质板中微管插入的位置。这些蛋白可能是微管帽结构的组成部分，并可能介导微管与肌动蛋白基质的相互作用。微管与角质板的结构相互作用为转导装置与毛细胞的轴向细胞骨架提供了重要的机械耦合。
