Currie P D, Sullivan D T
Department of Biology, Syracuse University, New York 13244-1270.
J Biol Chem. 1994 Oct 7;269(40):24679-87.
The gene from Drosophila melanogaster that encodes phosphofructokinase has been isolated and its structure and expression characterized. Southern blots of genomic DNA, comparison of multiple clones, and in situ hybridization to salivary gland chromosomes indicate that the Drosophila genome contains a single copy of the gene which encodes phosphofructokinase (PFK) that is located at cytogenetic position 46D/E on the polytene map. Analysis of the gene structure reveals a single coding region that spans 6.5 kilobase pairs and is composed of eight exons and seven introns. Three developmentally regulated transcripts are generated through the use of alternative polyadenylation sites. Transcription initiation occurs at a single site. The pattern of transcript accumulation during development is similar to other genes of Drosophila which encode enzymes of the glycolytic pathway. Deficiency analysis of the 46D/E region has identified a lethal complementation group associated with reduced PFK activity, thereby establishing the vital role of PFK function in Drosophila.
编码磷酸果糖激酶的黑腹果蝇基因已被分离出来，其结构和表达特征也已得到表征。基因组DNA的Southern杂交、多个克隆的比较以及对唾液腺染色体的原位杂交表明，果蝇基因组包含一个编码磷酸果糖激酶（PFK）的基因单拷贝，该基因位于多线图上的细胞遗传学位置46D/E处。对基因结构的分析揭示了一个跨越6.5千碱基对的单一编码区域，由八个外显子和七个内含子组成。通过使用不同的聚腺苷酸化位点产生了三种受发育调控的转录本。转录起始于单个位点。发育过程中转录本积累的模式与果蝇中其他编码糖酵解途径酶的基因相似。对46D/E区域的缺失分析确定了一个与PFK活性降低相关的致死互补群，从而确立了PFK功能在果蝇中的重要作用。
