黑腹果蝇磷酸果糖激酶的遗传与生化特性
Genetic and biochemical characterization of phosphofructokinase from Drosophila melanogaster.
作者信息
Munneke L R, Collier G E
出版信息
Biochem Genet. 1985 Dec;23(11-12):847-57. doi: 10.1007/BF00499933.
PMID:2935142
Abstract
Phosphofructokinase (PFK;EC 2.7.1.11) activity in Drosophila melanogaster is controlled by a single dosage-sensitive region of the genome between 45F and 47E of chromosome IIR. Only a single form of PFK was detected electrophoretically in both adults and larvae. Nearly 90% of the PFK activity in adults is localized to the thorax. Purification of the enzyme was hampered by the extreme lability of Drosophila PFK; however, a 36-fold partial purification was achieved.
摘要
果蝇（Drosophila melanogaster）中的磷酸果糖激酶（PFK；EC 2.7.1.11）活性受位于IIR染色体45F至47E之间基因组的一个单一剂量敏感区域控制。在成虫和幼虫中通过电泳仅检测到一种形式的PFK。成虫中近90%的PFK活性定位于胸部。果蝇PFK的极度不稳定阻碍了该酶的纯化；然而，实现了36倍的部分纯化。
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