Roselli-Rehfuss L, Ye F, Lissemore J L, Sullivan D T
Department of Biology, Syracuse University, New York 13244.
Mol Gen Genet. 1992 Nov;235(2-3):213-20. doi: 10.1007/BF00279363.
The gene that encodes phosphoglycerate kinase in Drosophila melanogaster (Pgk) has been isolated and characterized. There is a single copy of Pgk in the Drosophila genome located at cytogenetic position 23A1-2. Transcripts of Pgk are 1.6 kb long and are found during development with a profile similar to the expression pattern of other genes of the glycolytic pathway. There are substantial amounts of maternal transcript in early embryos which decline in abundance until mid-embryogenesis when transcript levels increase; levels remain high, during larval stages, fall during pupariation and rise again at emergence. The nucleotide sequence of the Pgk gene reveals two small introns, one of which is at a position identical to the site of an intron found in Pgk genes from other organisms. The Pgk gene has no TATA box in the region of transcription initiation and has multiple transcription initiation sites that are closely spaced within 110 nucleotides of the translation start site.
黑腹果蝇中编码磷酸甘油酸激酶（Pgk）的基因已被分离并进行了特征分析。果蝇基因组中Pgk只有一个拷贝，位于细胞遗传学位置23A1-2。Pgk的转录本长度为1.6 kb，在发育过程中被发现，其分布模式与糖酵解途径中其他基因的表达模式相似。早期胚胎中有大量母源转录本，其丰度会下降，直到胚胎发育中期转录本水平增加；在幼虫阶段水平保持较高，在化蛹时下降，并在羽化时再次上升。Pgk基因的核苷酸序列显示有两个小内含子，其中一个的位置与其他生物的Pgk基因中发现的一个内含子位点相同。Pgk基因在转录起始区域没有TATA框，并且有多个转录起始位点，它们在翻译起始位点的110个核苷酸内紧密排列。
