αvβ6整合素通过β6胞质结构域的一个独特区域促进结肠癌细胞的增殖。
The alpha v beta 6 integrin promotes proliferation of colon carcinoma cells through a unique region of the beta 6 cytoplasmic domain.
作者信息
Agrez M, Chen A, Cone R I, Pytela R, Sheppard D
机构信息
Department of Medicine, University of California, San Francisco.
出版信息
J Cell Biol. 1994 Oct;127(2):547-56. doi: 10.1083/jcb.127.2.547.
Abstract
Cell-matrix interactions are assumed to be important in regulating differentiation and tumor cell growth; however, the precise roles of individual matrix receptors in producing cellular responses are still unclear. We have previously described the alpha v beta 6 integrin, an epithelial cell fibronectin receptor expressed in many carcinoma cell lines. Here we show that heterologous expression of alpha v beta 6 in a human colon carcinoma cell line (SW480) enhances the proliferative capacity of these cells, both in vitro and in vivo in nude mice. This property of alpha v beta 6 correlates with the presence of an 11-amino acid region at the COOH terminus of the beta 6 cytoplasmic domain. This 11-amino acid sequence is required for the growth stimulatory effect, but not for other functions of the beta 6 cytoplasmic domain, such as promoting cell adhesion and focal contact localization.
摘要
细胞与基质的相互作用被认为在调节分化和肿瘤细胞生长中起重要作用；然而，单个基质受体在产生细胞反应中的精确作用仍不清楚。我们之前描述过αvβ6整合素，一种在许多癌细胞系中表达的上皮细胞纤连蛋白受体。在此我们表明，αvβ6在人结肠癌细胞系（SW480）中的异源表达增强了这些细胞在体外和裸鼠体内的增殖能力。αvβ6的这一特性与β6细胞质结构域COOH末端的一个11个氨基酸区域的存在相关。这一11个氨基酸序列是生长刺激效应所必需的，但不是β6细胞质结构域的其他功能所必需的，比如促进细胞黏附和粘着斑定位。
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