Kleter G A, Damen J J, Everts V, Niehof J, Ten Cate J M
Department of Cariology and Endodontology, Academic Centre for Dentistry (ACTA), Amsterdam, The Netherlands.
J Dent Res. 1994 Sep;73(9):1523-9. doi: 10.1177/00220345940730090701.
The effect of matrix degradation on the rate of demineralization of dentin lesions was investigated. It was hypothesized that the demineralized matrix would inhibit the demineralization of the underlying mineralized dentin. Bovine root dentin specimens were alternately demineralized and incubated with either a bacterial collagenase or buffer (control). The demineralization was carried out under various conditions: Acetic acid solutions were used to form incipient and advanced erosive lesions, and lactic acid solutions containing a bisphosphonate were used to form incipient subsurface lesions. Under all conditions, the demineralization was found to be accelerated when the matrix was degraded by collagenase. This increase was more pronounced in advanced erosive lesions than in incipient lesions. Microscopic examination of collagenase-treated specimens revealed that the matrix of erosive lesions contained several layers of differently affected matrices, whereas the matrix of subsurface lesions appeared to be equally affected throughout the lesion. In conclusion, the matrix degradation was different in erosive and subsurface lesions but promoted the demineralization in both types of lesions.
研究了基质降解对牙本质病变脱矿速率的影响。研究假设脱矿基质会抑制其下方矿化牙本质的脱矿。将牛牙根牙本质标本交替进行脱矿处理，并分别与细菌胶原酶或缓冲液（对照）孵育。脱矿在各种条件下进行：使用乙酸溶液形成早期和进展期侵蚀性病变，使用含有双膦酸盐的乳酸溶液形成早期潜行性病变。在所有条件下，当基质被胶原酶降解时，脱矿加速。这种增加在进展期侵蚀性病变中比在早期病变中更明显。对经胶原酶处理的标本进行显微镜检查发现，侵蚀性病变的基质包含几层受影响程度不同的基质，而潜行性病变的基质在整个病变中似乎受到同等程度的影响。总之，侵蚀性病变和潜行性病变中的基质降解情况不同，但均促进了两种类型病变的脱矿。
