Oral and Dental Disease Research Center, Department of Operative Dentistry, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Operative Dentistry, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
PLoS One. 2020 Sep 17;15(9):e0238590. doi: 10.1371/journal.pone.0238590. eCollection 2020.
The aim of this study was to evaluate the effect of silver diamine fluoride and grape seed extract on the microstructure and mechanical properties of carious dentin following exposure to acidic challenge. Ninety-eight molars with occlusal caries were used. In the control group the specimens were kept in distilled water. In the GSE group, the specimens were immersed in 6.5% grape seed extract solution for 30 minutes. In the SDF group, the specimens were immersed in 30% SDF solution for 4 minutes. In the GSE+SDF group, the specimens were immersed in 6.5% grape seed extract solution for 30 minutes and then exposed to 30% SDF solution for 4 minutes. All the groups underwent pH cycling model for 8 days. Microhardness measurements were taken at the baseline before surface treatments and after pH cycling. Elastic modulus was measured, after pH cycling. In the control group, the final hardness was significantly lower than the initial hardness (P = 0.001). In the SDF group, the final hardness was significantly higher than the initial hardness (P < 0.001). There was no significant difference between the initial and final hardness values in the GSE and GSE + SDF groups (p = 0.92, p = 0.07). The H1-H0 in the SDF group was significantly higher than the other groups (P<0.05). Moreover, elastic modulus of the experimental groups except GSE+SDF group was significantly higher than control. The highest mean elastic modulus was detected in the SDF group (P<0.001). The use of SDF and GSE prior to the acid challenge improved mechanical properties. Microstructural investigation, using scanning electron microscope showed dentin structure protection against acid challenges with SDF treatment and collagen matrix stabilization with GSE treatment. However combined use of these agents was not beneficious.
本研究旨在评估在酸性挑战后，银胺氟化物和葡萄籽提取物对龋坏牙本质微观结构和机械性能的影响。使用 98 颗磨牙进行研究。在对照组中，标本保持在蒸馏水中。在 GSE 组中，标本浸入 6.5%葡萄籽提取物溶液中 30 分钟。在 SDF 组中，标本浸入 30% SDF 溶液中 4 分钟。在 GSE+SDF 组中，标本先浸入 6.5%葡萄籽提取物溶液中 30 分钟，然后暴露于 30% SDF 溶液中 4 分钟。所有组均接受 pH 循环模型 8 天。在表面处理前和 pH 循环后进行显微硬度测量。在 pH 循环后测量弹性模量。在对照组中，最终硬度明显低于初始硬度（P=0.001）。在 SDF 组中，最终硬度明显高于初始硬度（P<0.001）。GSE 和 GSE+SDF 组的初始和最终硬度值之间无显著差异（p=0.92，p=0.07）。SDF 组的 H1-H0 明显高于其他组（P<0.05）。此外，实验组（除 GSE+SDF 组外）的弹性模量明显高于对照组。SDF 组的平均弹性模量最高（P<0.001）。在酸性挑战之前使用 SDF 和 GSE 可以改善机械性能。扫描电子显微镜的微观结构研究显示，SDF 处理可保护牙本质结构免受酸挑战，GSE 处理可稳定胶原基质。然而，联合使用这些药物没有益处。
