Naito M, Hayashi N, Hagiwara H, Katayama K, Kasahara A, Fusamoto H, Kato M, Masuzawa M, Kamada T
First Department of Medicine, Osaka University Medical School, Suita, Japan.
J Hepatol. 1994 Jun;20(6):755-9. doi: 10.1016/s0168-8278(05)80146-1.
To examine changes in the serum level of hepatitis C virus RNA in acute and chronic phases of hepatitis C virus infection, we tested serial serum samples of six patients with acute hepatitis C (posttransfusion: three; sporadic: three) and 11 patients with chronic hepatitis C using a competitive reverse transcription and polymerase chain reaction assay. The internal standard consisted of known amounts of synthetic mutated RNA. No patient with acute hepatitis showed resolution during the follow-up period (24-57 weeks). In posttransfusion cases, titers of hepatitis C virus RNA (log10[hepatitis C virus RNA copies/ml serum]) rose to a high level (7.5-9.5) in the early phase of infection (4-12 weeks after the transfusions) in association with the first serum alanine aminotransferase peaks. Titers of hepatitis C virus RNA then decreased, while serum alanine aminotransferase levels fluctuated with multiple peaks. In sporadic cases, titers of hepatitis C virus RNA had already reached a high level (7.0-7.5) at the first alanine aminotransferase peaks 2-3 weeks after the clinical onset. In chronic hepatitis C virus infection, titers of hepatitis C virus RNA remained high for follow-up periods of 6-12 years in patients with chronic active hepatitis. These results indicate that the replication of hepatitis C virus rose to a high level in the early phase of infection and that a high replicative level of hepatitis C virus might be related with progression of liver disease in the chronic phase of infection.
为检测丙型肝炎病毒感染急性期和慢性期血清丙型肝炎病毒RNA水平的变化，我们使用竞争性逆转录聚合酶链反应分析法检测了6例急性丙型肝炎患者（输血后：3例；散发性：3例）和11例慢性丙型肝炎患者的系列血清样本。内标由已知量的合成突变RNA组成。在随访期（24 - 57周）内，无急性肝炎患者病情缓解。在输血后病例中，丙型肝炎病毒RNA滴度（log10[丙型肝炎病毒RNA拷贝数/ml血清]）在感染早期（输血后4 - 12周）与首次血清丙氨酸氨基转移酶峰值相关，升至高水平（7.5 - 9.5）。随后丙型肝炎病毒RNA滴度下降，而血清丙氨酸氨基转移酶水平出现多个峰值波动。在散发性病例中，临床发病后2 - 3周首次丙氨酸氨基转移酶峰值时，丙型肝炎病毒RNA滴度已达到高水平（7.0 - 7.5）。在慢性丙型肝炎病毒感染中，慢性活动性肝炎患者的丙型肝炎病毒RNA滴度在6 - 12年的随访期内持续处于高水平。这些结果表明，丙型肝炎病毒复制在感染早期升至高水平，且丙型肝炎病毒的高复制水平可能与感染慢性期肝病的进展有关。
