Ochi Y, Koizumi T, Kobayashi S, Phuchareon J, Hatano M, Takada M, Tomita Y, Tokuhisa T
Department of Immunology, ICMR, Kobe University School of Medicine, Japan.
J Immunol. 1994 Oct 15;153(8):3485-90.
Effects of the deregulated c-fos gene product on IL-2 expression were studied in splenic T cells from c-fos transgenic (H2-c-fos) mice. IL-2 gene expression and IL-2 production by H2-c-fos T cells stimulated with immobilized anti-CD3 Abs were enhanced and prolonged as compared with those by control T cells. Activator protein-1 activity in nuclear extract from the H2-c-fos T cells was also higher than that from the control cells. There was no significant difference in the activity of other transcription factors including IL-2 kappa B, NFAT, and Oct-1, between the H2-c-fos and the control T cells. However, activity of a negative regulatory element binding factor (NRE-A) in the H2-c-fos T cells was much lower than that in the control cells. These results suggest that c-Fos/activator protein-1 is a major regulatory factor for IL-2 gene expression in splenic T cells activated through the TCR/CD3 complex.
在来自c-fos转基因（H2-c-fos）小鼠的脾T细胞中研究了失调的c-fos基因产物对白细胞介素-2（IL-2）表达的影响。与对照T细胞相比，用固定化抗CD3抗体刺激的H2-c-fos T细胞的IL-2基因表达和IL-2产生增强且延长。H2-c-fos T细胞核提取物中的激活蛋白-1活性也高于对照细胞。H2-c-fos T细胞和对照T细胞之间，包括IL-2κB、活化T细胞核因子（NFAT）和八聚体转录因子1（Oct-1）在内的其他转录因子的活性没有显著差异。然而，H2-c-fos T细胞中负调节元件结合因子（NRE-A）的活性远低于对照细胞。这些结果表明，c-Fos/激活蛋白-1是通过T细胞受体/CD3复合物激活的脾T细胞中IL-2基因表达的主要调节因子。
