Nadin-Davis S A, Casey G A, Wandeler A I
Animal Diseases Research Institute, Pathobiology Section, Agriculture Canada, Nepean, Ontario.
J Gen Virol. 1994 Oct;75 ( Pt 10):2575-83. doi: 10.1099/0022-1317-75-10-2575.
Rabies persists in Ontario wildlife in two predominant species: the red fox (Vulpes vulpes) and the striped skunk (Mephitis mephitis). A protocol applying reverse transcription/polymerase chain reaction (RT/PCR) and restriction endonuclease analysis (REA) to the rabies virus nucleoprotein gene was previously reported by Nadin-Davis et al. (Journal of General Virology 74, 829-837, 1993) to be useful for discrimination of rabies virus variants in Ontario. Four main types, which showed no host species specificity but which did exhibit different geographical distributions, were identified. Between 1989 and 1992 an area north and west of the city of North Bay experienced unusual and substantial rabies activity. In this report we describe the use of these molecular techniques to investigate the epidemiology of this recent rabies outbreak in central Ontario. It is shown that two of the four previously identified variants had invaded this region from the south and east, but in addition viruses very closely related to arctic isolates of rabies virus were found. The nucleoprotein and glycoprotein genes of this arctic type were sequenced and compared to those of its more southerly neighbours.
赤狐（赤狐属）和条纹臭鼬（臭鼬属）。Nadin-Davis等人（《普通病毒学杂志》74卷，829 - 837页，1993年）先前报道了一种将逆转录/聚合酶链反应（RT/PCR）和限制性内切酶分析（REA）应用于狂犬病病毒核蛋白基因的方案，该方案可用于区分安大略省的狂犬病病毒变种。已鉴定出四种主要类型，它们没有宿主物种特异性，但呈现出不同的地理分布。1989年至1992年期间，北湾市以北和以西的地区经历了异常且大量的狂犬病活动。在本报告中，我们描述了使用这些分子技术来调查安大略省中部近期狂犬病疫情的流行病学情况。结果表明，先前鉴定出的四种变种中的两种是从南部和东部侵入该地区的，但此外还发现了与狂犬病病毒北极分离株密切相关的病毒。对这种北极型病毒的核蛋白和糖蛋白基因进行了测序，并与其更靠南的邻近病毒的基因进行了比较。
