Fahrig T
Department of Experimental Neurology, Troponwerke GmbH & Co. KG, Cologne, Germany.
J Neurochem. 1994 Nov;63(5):1796-801. doi: 10.1046/j.1471-4159.1994.63051796.x.
In the present study, changes in the content of glial fibrillary acidic protein (GFAP) in mouse cortex were investigated at different time intervals after unilateral middle cerebral artery occlusion. The GFAP content was assessed semiquantitatively by ELISA and immunoblotting. GFAP immunoreactivity was determined for each animal separately in protein fractions obtained from the ipsilateral, lesioned cortex and the contralateral, unlesioned cortex. Changes in the GFAP content of the lesioned cortex with respect to that of the unlesioned cortex were calculated for each fraction individually. GFAP was detectable in all protein fractions with a significant amount recovered from the aqueous extracts. A pronounced increase in the GFAP content of the lesioned cortex was observed. As measured by ELISA, this increase was maximal 5 days after injury and significantly more pronounced for the soluble and the Triton X-100-soluble protein fractions (mean increase 7 days after lesion, 281.4 and 240.2%, respectively) than for the crude cytoskeletal fraction (mean increase, 153.3%). A small and transient increase in GFAP immunoreactivity was also found in all protein fractions prepared from the contralateral, unlesioned cortex. These results were confirmed by immunoblotting.
在本研究中，在单侧大脑中动脉闭塞后的不同时间间隔，研究了小鼠皮质中胶质纤维酸性蛋白（GFAP）含量的变化。通过酶联免疫吸附测定（ELISA）和免疫印迹法对GFAP含量进行半定量评估。对从同侧损伤皮质和对侧未损伤皮质获得的蛋白质组分中的每只动物分别测定GFAP免疫反应性。针对每个组分单独计算损伤皮质中GFAP含量相对于未损伤皮质中GFAP含量的变化。在所有蛋白质组分中均可检测到GFAP，且从水提取物中回收了大量的GFAP。观察到损伤皮质中GFAP含量显著增加。通过ELISA测量，这种增加在损伤后5天达到最大，并且可溶性和Triton X-100可溶性蛋白质组分（损伤后7天平均增加分别为281.4%和240.2%）的增加明显比粗细胞骨架组分（平均增加153.3%）更显著。在从对侧未损伤皮质制备的所有蛋白质组分中也发现GFAP免疫反应性有小的短暂增加。这些结果通过免疫印迹得到证实。
