Kim H, Ko J P, Kang U G, Park J B, Kim H L, Lee Y H, Kim Y S
Department of Anatomy, College of Medicine, Korea University, Seoul.
J Neurochem. 1994 Nov;63(5):1991-4. doi: 10.1046/j.1471-4159.1994.63051991.x.
We investigated the expression of inositol 1,4,5-trisphosphate (InsP3) 3-kinase mRNA after a single electroconvulsive shock (ECS) with in situ hybridization histochemistry in rat brain. At 6 h after ECS, the expression was markedly decreased in the dentate gyrus, and the decrease was maintained until 9 h with a slight recovery. The InsP3 3-kinase mRNA content returned to basal levels after 12 h. We could not detect any apparent changes in the expression of InsP3 3-kinase mRNA in the CA1-CA3 areas of hippocampus, the striatum, and the cerebral cortex at any time point examined. In the temporal pattern, the reduction of the expression in the dentate gyrus was preceded by the induction of c-fos after ECS. These observations suggest that the InsP3 3-kinase might be one of the genes whose expression can be altered by ECS.
我们采用原位杂交组织化学方法，研究了大鼠脑单次电惊厥休克（ECS）后肌醇1,4,5-三磷酸（InsP3）3-激酶mRNA的表达情况。ECS后6小时，齿状回中的表达显著降低，并持续至9小时，仅有轻微恢复。12小时后，InsP3 3-激酶mRNA含量恢复至基础水平。在任何检测时间点，我们均未在海马体CA1-CA3区、纹状体和大脑皮层中检测到InsP3 3-激酶mRNA表达的明显变化。在时间模式上，ECS后齿状回中表达的降低先于c-fos的诱导。这些观察结果表明，InsP3 3-激酶可能是其表达可被ECS改变的基因之一。
