Bikker P, Verstegen M W, Bosch M W
Department of Animal Nutrition, Agricultural University, Wageningen, The Netherlands.
J Nutr. 1994 Oct;124(10):1961-9. doi: 10.1093/jn/124.10.1961.
Ninety-five female pigs from 20 to 45 kg body weight were used to elucidate the effects of energy and protein intake on the amino acid composition of the protein in the carcass, organs and empty body of growing pigs. In a 2 x 15 factorial arrangement, protein intake ranged from 127 to 350 g/d in 15 graduated steps; and the digestible energy allowances were 15.8 and 18.8 MJ/d. Whole-body amino acid contents (g/16 g nitrogen) were (means +/- SEM) lysine 6.64 +/- 0.028, methionine 2.11 +/- 0.012; threonine 3.62 +/- 0.016 and total essential amino acids 42.8 +/- 0.16. The organ fraction contained 14.8 and 15.8% (SEM 0.13) of whole-body protein at the low and high energy levels, respectively. The concentrations of essential amino acids were 41.8 +/- 0.19 and 48.4 +/- 0.13 g/16 g nitrogen in the carcass and organs, respectively. Concentrations of a number of amino acids (in carcass, organ and whole-body protein and in protein deposited between 20 and 45 kg, were affected by protein and/or energy intake. The amino acid pattern of the newly deposited protein was slightly different from that of the empty body protein. The changes in amino acid contents were presumably the result of effects of protein and energy intake on the proportions of muscle and non-muscle carcass tissues and on relative weights of blood and viscera. Consequences of these changes for the amino acid requirements are discussed.
选用95头体重在20至45千克之间的雌性猪，以阐明能量和蛋白质摄入量对生长猪胴体、器官及空体蛋白质氨基酸组成的影响。采用2×15析因设计，蛋白质摄入量以15个梯度递增，范围为127至350克/天；可消化能量供给分别为15.8和18.8兆焦/天。全身氨基酸含量（克/16克氮）为（平均值±标准误）：赖氨酸6.64±0.028、蛋氨酸2.11±0.012、苏氨酸3.62±0.016，必需氨基酸总量42.8±0.16。在低能量和高能量水平下，器官部分分别占全身蛋白质的14.8%和15.8%（标准误0.13）。胴体和器官中必需氨基酸浓度分别为41.8±0.19和48.4±0.13克/16克氮。蛋白质和/或能量摄入量会影响许多氨基酸在胴体、器官和全身蛋白质以及20至45千克体重期间沉积蛋白质中的浓度。新沉积蛋白质的氨基酸模式与空体蛋白质略有不同。氨基酸含量的变化可能是蛋白质和能量摄入量对肌肉和非肌肉胴体组织比例以及血液和内脏相对重量产生影响的结果。讨论了这些变化对氨基酸需求的影响。
