Clayden A M, Young W G, Zhang C Z, Harbrow D, Romaniuk K, Waters M J
Faculty of Dentistry, University of Queensland, Australia.
J Periodontal Res. 1994 Jul;29(4):266-75. doi: 10.1111/j.1600-0765.1994.tb01221.x.
To document the effect of hypophysectomy and growth hormone replacement on the ultrastructure of cementogenesis in the developing rat third molar, 12 female Wistar rats were randomly allocated to normal control, hypophysectomized or hypophysectomized plus human growth hormone (for 10 days) treatment groups. The results of this study by electron and light microscopy and morphometry have shown that qualitative and quantitative changes occur in the organelles of cementoblasts forming cellular cementum as a result of hypophysectomy and growth hormone replacement. After hypophysectomy, the changes of less prominent nucleoli and nuclear pores, less prominent Golgi apparatuses and decreased endoplasmic reticulum can be interpreted as diminished cementum matrix biosynthesis--an interpretation that can be confirmed morphometrically by less cellular cementum formation. Growth hormone replacement for 10 days reactivates protein synthesis and cementogenesis as evidenced by ultrastructural changes in cementoblasts and a greater production of cementum.
为记录垂体切除及生长激素替代对发育中大鼠第三磨牙牙骨质生成超微结构的影响，将12只雌性Wistar大鼠随机分为正常对照组、垂体切除组或垂体切除加人生长激素（持续10天）治疗组。这项通过电子显微镜、光学显微镜及形态测量学进行的研究结果表明，垂体切除及生长激素替代导致形成细胞性牙骨质的成牙骨质细胞细胞器出现质和量的变化。垂体切除后，核仁及核孔不那么明显、高尔基体不那么突出以及内质网减少等变化可解释为牙骨质基质生物合成减少——这一解释可通过细胞性牙骨质形成减少的形态测量结果得到证实。持续10天的生长激素替代可重新激活蛋白质合成及牙骨质生成，这在成牙骨质细胞的超微结构变化及更多牙骨质生成中得到了体现。
