Power W J, Neylan D, Collum L M
Department of Ophthalmology, Royal Victoria Eye and Ear Hospital, Dublin, Ireland.
J Cataract Refract Surg. 1994 Jul;20(4):440-5. doi: 10.1016/s0886-3350(13)80181-2.
We developed an in vitro model to assess the adherence of human lens epithelial cells to three types of intraocular lenses: poly(methyl methacrylate) (PMMA), heparin-surface-modified PMMA (HSM-PMMA), and polyHema. Lenses were incubated with a fixed number of human lens epithelial cells. Adherent cells were counted after 72 hours in culture. Scanning electron microscopy showed significantly fewer cells adhering to the HSM-PMMA and polyHema lenses than to the PMMA lenses (P < .01). Repeat experiments on cell lines established from different donors confirmed these findings.
我们开发了一种体外模型，以评估人晶状体上皮细胞对三种类型人工晶状体的黏附情况：聚甲基丙烯酸甲酯（PMMA）、肝素表面修饰的PMMA（HSM-PMMA）和聚甲基丙烯酸羟乙酯（polyHema）。将人工晶状体与固定数量的人晶状体上皮细胞一起孵育。培养72小时后对黏附细胞进行计数。扫描电子显微镜显示，与PMMA人工晶状体相比，黏附在HSM-PMMA和polyHema人工晶状体上的细胞明显更少（P <.01）。对来自不同供体建立的细胞系进行的重复实验证实了这些发现。
