Chen Y P, Johnson G K, Squier C A
Dows Institute for Dental Research, University of Iowa, Iowa City 52242-1010.
J Oral Pathol Med. 1994 Jul;23(6):251-5. doi: 10.1111/j.1600-0714.1994.tb00054.x.
Fifty-two hamsters were divided into 6 groups and their cheek pouches treated with either 0.01% NNN, 0.01% NNN and 6% nicotine, 0.01% NNK, 0.01% NNK and 6% nicotine, 6% nicotine, or sesame oil. After painting each pouch 3 times per week for 13 months, the animals were killed and specimens from the cheek pouch and forestomach examined. Cheek pouch epithelium showed more frequent histologic changes, including hyperplasia, hyperkeratosis and, in one animal, moderate dysplasia, when nicotine was combined with NNN than after treatment with NNN (or nicotine) alone. There was a higher frequency of hyperplasia with hyperkeratosis in the forestomach when nicotine was combined with NNK than following NNK, or nicotine treatment alone, and squamous cell papillomas were evident in animals treated with both NNK and nicotine. These results suggest that in mucosal tissues nicotine may enhance the effect of weak carcinogens such as the nitrosamines.
52只仓鼠被分成6组，其颊囊分别用0.01%的NNN、0.01%的NNN和6%的尼古丁、0.01%的NNK、0.01%的NNK和6%的尼古丁、6%的尼古丁或芝麻油处理。每周对每个颊囊涂抹3次，持续13个月后，处死动物并检查颊囊和前胃的标本。与单独用NNN（或尼古丁）处理相比，当尼古丁与NNN联合使用时，颊囊上皮出现更频繁的组织学变化，包括增生、角化过度，并且在一只动物中出现了中度发育异常。当尼古丁与NNK联合使用时，前胃中增生伴角化过度的频率高于单独使用NNK或尼古丁处理后，并且在同时接受NNK和尼古丁处理的动物中可见鳞状细胞乳头状瘤。这些结果表明，在黏膜组织中，尼古丁可能会增强亚硝胺等弱致癌物的作用。
