与亚致癌剂量的4-硝基喹啉-N-氧化物和7,12-二甲基苯并(a)蒽联合使用时，N-亚硝基降烟碱的促癌作用。

The cancer-promoting effect of N-nitrosonornicotine used in combination with a subcarcinogenic dose of 4-nitroquinoline-N-oxide and 7,12-dimethylbenz (A) anthracene.

作者信息

Altuwairgi O S, Papageorge M B, Doku H C

机构信息

Department of Oral and Maxillofacial Surgery, Tufts University School of Dental Medicine, Boston, MA 02111, USA.

出版信息

J Oral Maxillofac Surg. 1995 Aug;53(8):910-3; discussion 914. doi: 10.1016/0278-2391(95)90279-1.

DOI:10.1016/0278-2391(95)90279-1

PMID:7629619

Abstract

PURPOSE

This study was conducted to determine the possible carcinogenic role of N-Nitrosonornicotine (NNN) when combined with subcarcinogenic doses of strong carcinogens dimethylbenz (a) anthracene (DMBA) and 4-nitroquinoline-N-oxide (4NQO) in the hamster cheek pouch.

MATERIALS AND METHODS

Eighty-five Syrian golden hamsters were randomly divided into three main groups. Group A contained 35 animals, 20 of which were treated with 0.1% DMBA followed by 4% NNN (A-I), 5 with 0.1% DMBA (A-II), 5 with 4% NNN (A-III), and 5 with mineral oil alone (A-IV). Group B contained 23 animals, 13 of which were treated with 0.5% 4NQO followed by 4% NNN (B-I), 5 with 0.5% 4NQO (B-II), and 5 animals with propyleneglycol alone (B-III). Group C contained 27 animals, 14 of which were treated with 0.1% DMBA followed by 4% NNN and 0.5% 4NQC (C-I), and 13 with 0.1% DMBA followed by 0.5% 4NQO (C-II). All animals were treated three times per week for 16 weeks. A total of 7 animals died during this period.

RESULTS

Squamous cell carcinoma (SCCA) developed in eight animals (67%) in the group treated with all three chemicals (C-I), in four animals (33%) treated with DMBA and 4NQO (C-II), in two animals (15%) treated with 4NQO and NNN (B-I), and in two animals (11%) treated with DMBA and NNN (A-I). The difference between the number of animals that developed carcinoma in group C-I and those in groups A-I and B-I was statistically significant (P < .05) and this difference reached a significant value when group C-I and C-II were compared (P < or = .1). There was a direct relationship between the number of tumors produced in animals and the number of different chemicals applied.

CONCLUSION

The results of this study indicate that NNN, when combined with subcarcinogenic doses of other strong carcinogens, is a promoter in the development of squamous cell carcinoma and that 4NQO in 0.5% concentration is a stronger carcinogen than 0.1% DMBA.

摘要

目的

本研究旨在确定N-亚硝基降烟碱（NNN）与亚致癌剂量的强致癌物二甲基苯并（a）蒽（DMBA）和4-硝基喹啉-N-氧化物（4NQO）联合作用于仓鼠颊囊时可能的致癌作用。

材料与方法

85只叙利亚金仓鼠随机分为三个主要组。A组有35只动物，其中20只先用0.1% DMBA处理，随后用4% NNN处理（A-I），5只只用0.1% DMBA处理（A-II），5只只用4% NNN处理（A-III），5只只用矿物油处理（A-IV）。B组有23只动物，其中13只先用0.5% 4NQO处理，随后用4% NNN处理（B-I），5只只用0.5% 4NQO处理（B-II），5只只用丙二醇处理（B-III）。C组有27只动物，其中14只先用0.1% DMBA处理，随后用4% NNN和0.5% 4NQC处理（C-I），13只先用0.1% DMBA处理，随后用0.5% 4NQO处理（C-II）。所有动物每周处理3次，共处理16周。在此期间共有7只动物死亡。

结果

在接受所有三种化学物质处理的组（C-I）中，8只动物（67%）发生了鳞状细胞癌（SCCA）；在接受DMBA和4NQO处理的组（C-II）中，4只动物（33%）发生了SCCA；在接受4NQO和NNN处理的组（B-I）中，2只动物（15%）发生了SCCA；在接受DMBA和NNN处理的组（A-I）中，2只动物（11%）发生了SCCA。C-I组发生癌的动物数量与A-I组和B-I组之间的差异具有统计学意义（P <.05），当比较C-I组和C-II组时，这种差异达到显著水平（P <或=.1）。动物产生的肿瘤数量与应用的不同化学物质数量之间存在直接关系。