四环素对复发性阿弗他溃疡患者胶原酶活性的影响。

Effect of tetracyclines on collagenase activity in patients with recurrent aphthous ulcers.

作者信息

Häyrinen-Immonen R, Sorsa T, Pettilä J, Konttinen Y T, Teronen O, Malmström M

机构信息

Department of Oral and Maxillofacial Surgery, University of Helsinki, Finland.

出版信息

J Oral Pathol Med. 1994 Jul;23(6):269-72. doi: 10.1111/j.1600-0714.1994.tb00057.x.

DOI:10.1111/j.1600-0714.1994.tb00057.x

PMID:7932246

Abstract

Human neutrophil-type (MMP-8) and fibroblast-type (MMP-1) interstitial collagenase, and their inhibition by tetracyclines in saliva from patients with recurrent aphthous ulcers (RAU) or aphthae, were studied by means of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and enzymological analyses. In the salivary specimens obtained from patients with aphthae, collagenase was found in endogenously active form and was predominantly of MMP-8 type. Topical rinsing treatment with chlortetracycline (Aureomycin) alleviated the discomfort caused by the lesions but did not reduce salivary collagenase amounts; however in vitro, doxycycline inhibited salivary collagenase totally.

摘要

通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）和酶学分析，研究了人中性粒细胞型（MMP-8）和成纤维细胞型（MMP-1）间质胶原酶，以及四环素对复发性阿弗他溃疡（RAU）或口疮患者唾液中这些酶的抑制作用。在口疮患者的唾液标本中，发现胶原酶以内源活性形式存在，且主要为MMP-8型。用金霉素局部冲洗治疗可减轻病变引起的不适，但并未降低唾液中胶原酶的含量；然而在体外，强力霉素可完全抑制唾液中的胶原酶。