Van Haaster L H, De Rooij D G
Department of Cell Biology, Utrecht University, Medical School, The Netherlands.
J Reprod Fertil. 1994 Jul;101(2):321-6. doi: 10.1530/jrf.0.1010321.
The frequencies of the cellular associations of the seminiferous epithelium were determined at various ages after birth in immature Djungarian hamsters and Wistar rats. The frequencies of the cellular associations present in immature animals were then compared with the frequencies of the corresponding pooled stages in adult animals. At 15 days of age, the cellular associations present in Djungarian hamsters could be divided into four groups based on the presence of A3, intermediate (In), or B (B1 and B2) spermatogonia, or preleptotene-leptotene-zygotene spermatocytes. Compared with adult animals, the group containing spermatocytes was found to be enriched by 52%, while the frequency of the B spermatogonia was reduced by 55%. At 22 days of age, the cellular associations of spermatogenesis were classified into five groups: pachytene spermatocytes associated with A3 spermatogonia. In spermatogonia, B spermatogonia, or preleptotene-leptotene-zygotene spermatocytes, or the meiotic divisions. The stages containing preleptotene-leptotene-zygotene spermatocytes and those containing A3 spermatogonia were enriched by 35% and 59% compared with adult values, respectively. The meiotic divisions were enriched by 21%, but this increase was not significant. The frequency of the B spermatogonia was reduced by 62%. In addition, the synchronization factor, calculated with the computer program SYNTEST, was increased to 1.48 +/- 0.05 (mean +/- SEM) and 1.38 +/- 0.03 at 15 and 22 days of age, respectively, and was significantly higher (P < 0.001 at both ages) than the adult value of 1.08 +/- 0.02. However, after 22 days of age the synchronization factor did not differ from that in adult animals.(ABSTRACT TRUNCATED AT 250 WORDS)
在出生后的不同年龄段，对未成熟的侏儒仓鼠和Wistar大鼠的生精上皮细胞组合频率进行了测定。然后将未成熟动物中存在的细胞组合频率与成年动物相应合并阶段的频率进行比较。在15日龄时，根据是否存在A3、中间型（In）或B（B1和B2）精原细胞，或前细线期 - 细线期 - 偶线期精母细胞，侏儒仓鼠中存在的细胞组合可分为四组。与成年动物相比，含有精母细胞的组增加了52%，而B精原细胞的频率降低了55%。在22日龄时，精子发生的细胞组合分为五组：粗线期精母细胞与A3精原细胞、In精原细胞、B精原细胞或前细线期 - 细线期 - 偶线期精母细胞相关联，或减数分裂。与成年值相比，含有前细线期 - 细线期 - 偶线期精母细胞的阶段和含有A3精原细胞的阶段分别增加了35%和59%。减数分裂增加了21%，但这种增加不显著。B精原细胞的频率降低了62%。此外，使用计算机程序SYNTEST计算的同步因子在15日龄和22日龄时分别增加到1.48±0.05（平均值±标准误）和1.38±0.03，并且显著高于成年值1.08±0.02（两个年龄段P均<0.001）。然而，22日龄后同步因子与成年动物的同步因子没有差异。（摘要截断于250字）
