van Haaster L H, De Rooij D G
Department of Cell Biology, Utrecht University, Medical School, The Netherlands.
Biol Reprod. 1993 Mar;48(3):515-21. doi: 10.1095/biolreprod48.3.515.
In this study, spermatogenesis in the adult Djungarian hamster is described. Undifferentiated spermatogonia topographically arranged as Asingle (A(s)), Apaired (Apr), and Aaligned (Aal) spermatogonia were observed, as were six generations of differentiating spermatogonia (A1, A2, A3, intermediate, B1, and B2). The differentiating spermatogonia divided at regular intervals during the cycle of the seminiferous epithelium. Mitosis of these cells was observed at the transition from stage IX to stage X (mitosis of A1 into A2 spermatogonia), at the transition from stage XII to stage I, at the transition from stage II to stage III, at the transition from stage IV to stage V, at the end of stage VI, and at approximately the middle of stage VII. Cellular associations in the cycle of the seminiferous epithelium are described. The seminiferous epithelium was divided into 12 stages, based upon the developmental steps in spermiogenesis, and the frequency of these stages was determined. The duration of the cycle of the seminiferous epithelium, determined by [3H]thymidine incorporation, was shown to be 7.90 +/- 0.01 (mean +/- SEM) days.
在本研究中，描述了成年侏儒仓鼠的精子发生过程。观察到未分化的精原细胞在地形上排列为单个A（A(s)）、配对A（Apr）和成排A（Aal）精原细胞，以及六代分化中的精原细胞（A1、A2、A3、中间型、B1和B2）。分化中的精原细胞在生精上皮周期中定期分裂。在从IX期到X期的转变（A1精原细胞分裂为A2精原细胞）、从XII期到I期的转变、从II期到III期的转变、从IV期到V期的转变、VI期末以及大约VII期中期观察到这些细胞的有丝分裂。描述了生精上皮周期中的细胞组合。根据精子发生的发育步骤，将生精上皮分为12个阶段，并确定了这些阶段的频率。通过[3H]胸苷掺入法确定的生精上皮周期持续时间为7.90 +/- 0.01（平均值 +/- 标准误）天。
