Ono T, Hirota K, Nemoto K, Fernandez E J, Ota F, Fukui K
Department of Oral Microbiology and Immunology, School of Dentistry, University of Tokushima, Japan.
J Med Microbiol. 1994 Oct;41(4):231-5. doi: 10.1099/00222615-41-4-231.
Synthetic oligonucleotide primers were used in the polymerase chain reaction (PCR) to amplify a sequence of the spaP gene, which encodes the surface protein antigen I/II of Streptococcus mutans. A DNA fragment of c. 192 bp was amplified from lysed S. mutans cells or isolated DNA. With S. mutans cells, the lower limit of detection was 4-40 cfu. With these primers, 13 reference and 50 clinical strains of S. mutans were identified. Amplification of the 192-bp product was not demonstrated when 41 strains of other streptococcal and non-streptococcal species were tested. The spaP gene PCR has potential for the rapid diagnosis of S. mutans infections.
在聚合酶链反应（PCR）中使用合成寡核苷酸引物来扩增spaP基因的一段序列，该基因编码变形链球菌的表面蛋白抗原I/II。从裂解的变形链球菌细胞或分离的DNA中扩增出约192 bp的DNA片段。对于变形链球菌细胞，检测下限为4 - 40 cfu。使用这些引物，鉴定出了13株变形链球菌参考菌株和50株临床菌株。当检测41株其他链球菌和非链球菌物种时，未显示出192 bp产物的扩增。spaP基因PCR在快速诊断变形链球菌感染方面具有潜力。
