Brady L J, Crowley P J, Ma J K, Kelly C, Lee S F, Lehner T, Bleiweis A S
Department of Oral Biology, University of Florida, Gainesville 32610.
Infect Immun. 1991 May;59(5):1803-10. doi: 10.1128/iai.59.5.1803-1810.1991.
A restriction fragment length polymorphism study was undertaken to determine the extent and location of heterogeneity within spaP encoding the Mr 185,000 cell surface protein P1 (antigen I/II) of Streptococcus mutans serotype c isolates. The gene was found to be highly conserved except for a central variable (V) region predicted to encode less than 150 amino acids. Sequence analysis identified two V-region variants. These differences were independent of the geographic source of the isolates. Southern analysis using synthetic oligonucleotide probes indicated that nonretention of P1 (I/II) by some isolates is not due to a deletion of the 3'-terminal DNA necessary to encode an intact carboxy terminus.
开展了一项限制性片段长度多态性研究，以确定变形链球菌c血清型分离株中编码185,000 Mr细胞表面蛋白P1（抗原I/II）的spaP基因内异质性的程度和位置。发现该基因高度保守，除了预测编码少于150个氨基酸的中央可变（V）区域。序列分析鉴定出两个V区域变体。这些差异与分离株的地理来源无关。使用合成寡核苷酸探针进行的Southern分析表明，一些分离株不保留P1（I/II）并非由于缺失编码完整羧基末端所需的3'末端DNA。
