van Besouw N M, van der Meide P H, Bakker N P
Department of Chronic and Infectious Diseases, Institute for Applied Radiobiology and Immunology (ITRI)-TNO, Rijswijk, The Netherlands.
J Med Primatol. 1994 Jan;23(1):42-8. doi: 10.1111/j.1600-0684.1994.tb00094.x.
An enzyme-linked immunoabsorbent spot (ELISPOT) assay has been developed for measuring the frequency of interferon-gamma (IFN-gamma) producing cells in rhesus monkeys. Aged monkeys revealed, upon mitogenic stimulation, a significantly higher percentage of IFN-gamma secreting peripheral blood mononuclear cells (PBMC) compared to young animals. No correlation was found between the frequency of IFN-gamma producing PBMC and the mitogen-driven proliferation, indicating that in rhesus monkeys no direct correlation exists between these two activation parameters.
已开发出一种酶联免疫斑点（ELISPOT）测定法，用于测量恒河猴中产生γ干扰素（IFN-γ）的细胞频率。与年轻动物相比，经丝裂原刺激后，老年猴分泌IFN-γ的外周血单个核细胞（PBMC）百分比显著更高。未发现产生IFN-γ的PBMC频率与丝裂原驱动的增殖之间存在相关性，这表明在恒河猴中，这两个激活参数之间不存在直接相关性。
