大肠杆菌赖氨酰 - tRNA合成酶（LysU）的结晶及初步衍射研究

Crystallization and preliminary diffraction studies of Escherichia coli lysyl-tRNA synthetase (LysU).

作者信息

Onesti S, Theoclitou M E, Pernilla E, Wittung L, Miller A D, Plateau P, Blanquet S, Brick P

机构信息

Blackett Laboratory, Imperial College London, England.

出版信息

J Mol Biol. 1994 Oct 14;243(1):123-5. doi: 10.1006/jmbi.1994.1635.

DOI:10.1006/jmbi.1994.1635

PMID:7932734

Abstract

Crystals of Escherichia coli lysyl-tRNA synthetase (lysU gene product) have been obtained by vapour diffusion techniques. Three different crystal forms could be grown under similar conditions. The crystals that have been chosen for the structure determination belong to space group C222(1) with cell dimensions a = 144.3 A, b = 257.8 A, c = 182.1 A and contain three monomers in the asymmetric unit. They diffract to at least 2.1 A resolution, but are very sensitive to radiation damage.

摘要

通过气相扩散技术获得了大肠杆菌赖氨酰 - tRNA合成酶（lysU基因产物）的晶体。在相似条件下可以生长出三种不同的晶体形式。已选择用于结构测定的晶体属于空间群C222(1)，晶胞参数为a = 144.3 Å，b = 257.8 Å，c = 182.1 Å，不对称单元中含有三个单体。它们的衍射分辨率至少为2.1 Å，但对辐射损伤非常敏感。