来自大肠杆菌的乳清酸磷酸核糖基转移酶脱辅基形式的结晶及初步X射线衍射研究。

Crystallization and preliminary X-ray diffraction studies on the Apo form of orotate phosphoribosyltransferase from Escherichia coli.

作者信息

Aghajari N, Jensen K F, Gajhede M

机构信息

Center for Crystallographic Studies, University of Copenhagen, Denmark.

出版信息

J Mol Biol. 1994 Aug 12;241(2):292-4. doi: 10.1006/jmbi.1994.1503.

DOI:10.1006/jmbi.1994.1503

PMID:8057372

Abstract

Three different crystal forms of the apoenzyme orotate phosphoribosyltransferase, with M(r) = 23,552 from Escherichia coli have been grown. The crystals, which are all suitable for X-ray diffraction analysis, have been grown by the hanging drop vapour diffusion method. The first form crystallizes in the orthorhombic space group P2(1)2(1)2, with cell dimensions: a = 136.34 A, b = 75.98 A and c = 40.32 A; the second form in the monoclinic space group P2, with unit cell dimensions: a = 101.61 A, b = 40.49 A, c = 79.05 A and beta = 87.33 degrees; and the third form in P2(1)2(1)2(1), the cell dimensions being a = 70.27 A, b = 103.53 A, c = 53.83 A.

摘要

已培养出大肠杆菌中分子量为23,552的脱辅基酶乳清酸磷酸核糖基转移酶的三种不同晶体形式。这些晶体均适用于X射线衍射分析，是通过悬滴气相扩散法培养出来的。第一种形式在正交空间群P2(1)2(1)2中结晶，晶胞尺寸为：a = 136.34 Å，b = 75.98 Å，c = 40.32 Å；第二种形式在单斜空间群P2中，晶胞尺寸为：a = 101.61 Å，b = 40.49 Å，c = 79.05 Å，β = 87.33°；第三种形式在P2(1)2(1)2(1)中，晶胞尺寸为a = 70.27 Å，b = 103.53 Å，c = 53.83 Å。