Wang P Y, Zhen T M, Wang Z Z, Gu Z F, Ren S P, Liu L H, Hou L W, Liu J L
Shandong Institute of Parasitic Diseases, Jining, PR China.
J Trop Med Hyg. 1994 Oct;97(5):269-76.
This paper reports the results of 10 years of observations on the clinical manifestations, pathology and immunity to filariasis and aetiological biology of filariae in three volunteers (first author and his family members) who were inoculated experimentally with infective larvae of periodic Brugia malayi in 1981. The changes in clinical symptoms and signs were recorded systematically. Microfilariae were first detected at 41 and 46 weeks after inoculation in two subjects and remained detectable in small numbers until 8-8.5 years after infection. The microfilarial density fluctuated at 1-2 mf 120 microliters-1. Thereafter no microfilariae were detected in 12 blood sample examinations, suggesting that the adult reproductive period of periodic B. malayi could last up to 8-9 years in the human body. Eosinophilia occurred mainly before and at the initial stage of microfilaraemia. An increase in the lymphocytes was observed to some extent at 2-156 weeks after infection. Biopsy at the inoculation site 6 weeks after inoculation showed infiltration of the lymph node by inflammatory cells, mainly eosinocytes, lymphocytes and monocytes. Lymphangiectasis and lymphostasis were observed in both limbs and pelvic regions by lymphangiogram taken 11 weeks after inoculation. Antibodies against B. malayi first appeared at 2-5 weeks after infection, peaked at 12-56 weeks and thereafter declined gradually. Subjects A and C became antibody free but subject B remained positive to antibody against B. malayi 10 years after infection. E-rosette forming lymphocytes became lower than normal at 11 weeks and recovered to normal within 10 years after infection.
本文报告了对三名志愿者（第一作者及其家庭成员）进行为期10年的观察结果，这些志愿者于1981年经实验接种了周期型马来布鲁线虫感染性幼虫，观察内容包括丝虫病的临床表现、病理学、免疫情况以及丝虫的病原生物学。系统记录了临床症状和体征的变化。两名受试者在接种后41周和46周首次检测到微丝蚴，并且在感染后8 - 8.5年一直能检测到少量微丝蚴。微丝蚴密度波动在1 - 2条/120微升。此后在12次血液样本检查中均未检测到微丝蚴，这表明周期型马来布鲁线虫在人体中的成虫生殖期可持续长达8 - 9年。嗜酸性粒细胞增多主要发生在微丝蚴血症之前及初期。感染后2 - 156周观察到淋巴细胞有一定程度增加。接种后6周在接种部位活检显示淋巴结有炎性细胞浸润，主要为嗜酸性粒细胞、淋巴细胞和单核细胞。接种后11周通过淋巴管造影观察到双下肢及盆腔区域淋巴管扩张和淋巴淤积。抗马来布鲁线虫抗体在感染后2 - 5周首次出现，在12 - 56周达到峰值，此后逐渐下降。受试者A和C在感染10年后抗体消失，但受试者B在感染10年后抗马来布鲁线虫抗体仍为阳性。E花环形成淋巴细胞在11周时低于正常水平，感染后10年内恢复正常。
