Liu J Y, Wang G Q, Chen Y N, Tu Z P, Fang Z
Fujian Provincial Institute of Parasitic Disease, Fuzhou, PR China.
J Trop Med Hyg. 1989 Apr;92(2):93-6.
This paper reports clinical observations made during experimental Brugia malayi infection in man. This was induced by 52 infective larvae-carrying mosquitoes, Anopheles sinensis. A monkey, Maccaca mulatta, was inoculated with infective larvae from the mosquitoes infected with nocturnally periodic type of B. malayi from patients. Six months later, the monkey became a microfilaria carrier. Nine days later, the subject was experimentally bitten by mosquitoes infected from biting the monkey, with an estimated delivery of over 200 infective larvae. Seven days after the infection, general (but fluctuating) pruritus developed, followed by lymphadenitis and swelling of the hand and forearm, with a mild pyrexia for nearly 3 months. The eosinophil counts continuously increased. There were four separate attacks of lymphangitis. On day 142, microfilaraemia was first detected. After two more weeks, the subject was treated with a total dose of 15 g diethylcarbamazine (DEC) over 30 days. During this period, 28 small tender subcutaneous nodules appeared on his arms. After treatment, microfilaraemia gradually disappeared and the swelling of his arms improved. Three months later, the eosinophil count of his blood returned to normal but some temporary and scattered small pruritic erythematous patches still persisted over his left forearm. Lymphangitis has not recurred.
本文报告了在人体实验性马来布鲁线虫感染期间所做的临床观察。感染由52只携带感染性幼虫的中华按蚊引发。一只恒河猴接种了来自感染了患者夜现周期性类型马来布鲁线虫的蚊子的感染性幼虫。六个月后，这只猴子成为了微丝蚴携带者。九天后，受试者被从叮咬猴子而感染的蚊子实验性叮咬，估计有超过200条感染性幼虫被传播。感染七天后，出现全身性（但有波动）瘙痒，随后是淋巴结炎以及手部和前臂肿胀，伴有近三个月的低热。嗜酸性粒细胞计数持续增加。有四次单独的淋巴管炎发作。在第142天，首次检测到微丝蚴血症。再过两周后，受试者在30天内接受了总量为15克的乙胺嗪（DEC）治疗。在此期间，他的手臂上出现了28个小的压痛性皮下结节。治疗后，微丝蚴血症逐渐消失，手臂肿胀有所改善。三个月后，他血液中的嗜酸性粒细胞计数恢复正常，但左前臂仍有一些暂时的、散在的小瘙痒性红斑。淋巴管炎未再复发。
