Lechín F, Coll-García E, Van Der Dijs B, Peña F, Bentolila A, Rivas C
Acta Physiol Lat Am. 1975;25(4):339-46.
The present study was aimed at observing the effect on insulin secretion of serotonin (5-HT) administered intraportally to anesthetized adult dogs. The influence of 5-HT on insulin release was also studied in mouse pancreatic islets isolated by a collagenase method. In the in vivo studies, 6 mg of 5-HT rapidly injected in the portal vein of dogs induced hypoglycemia, and a significant increase of immunoreactive insulin plasma levels (IRI) in blood samples taken from the pancreatoduodenal vein. The phenomenon was registered throughout three consecutive 10 min periods after serotonin administration. With 3 mg of 5-HT the IRI increases were not observed. When serotonin was slowly infused at doses of 3 and 6 mg, no increases of IRI were recorded. In the in vitro studies, 5-HT at 100 mug/ml stimulated the output of insulin in the presence of a low concentration of glucose (0.6 mg/ml); when the islets were incubated with glucose at a higher concentration (3.0 mg/ml) there was a lower insulin release in the presence of serotonin (100 mug/ml) than that obtained with glucose alone at the same concentration (3.0 mug/ml). The results obtained suggest that serotonin stimulates insulin release under certain conditions in the intact dog and also in the isolated pancreatic islets of the mouse incubated in vitro.
本研究旨在观察门静脉注射血清素（5-羟色胺，5-HT）对麻醉成年犬胰岛素分泌的影响。还研究了5-HT对用胶原酶法分离的小鼠胰岛胰岛素释放的影响。在体内研究中，向犬门静脉快速注射6mg 5-HT可导致低血糖，并使取自胰十二指肠静脉的血样中免疫反应性胰岛素血浆水平（IRI）显著升高。血清素给药后的三个连续10分钟期间均出现了这种现象。注射3mg 5-HT时未观察到IRI升高。当以3mg和6mg的剂量缓慢输注血清素时，未记录到IRI升高。在体外研究中，100μg/ml的5-HT在低浓度葡萄糖（0.6mg/ml）存在的情况下刺激胰岛素分泌；当胰岛与较高浓度（3.0mg/ml）的葡萄糖一起孵育时，在5-HT（100μg/ml）存在的情况下胰岛素释放低于相同浓度（3.0mg/ml）单独使用葡萄糖时的释放量。所得结果表明，血清素在完整犬的某些条件下以及体外培养的小鼠分离胰岛中均可刺激胰岛素释放。
