野生狐狸（东北狐）和蜱虫体内的莱姆病螺旋体。

Lyme disease spirochetes in a wild fox (Vulpes vulpes schrencki) and in ticks.

作者信息

Isogai E, Isogai H, Kawabata H, Masuzawa T, Yanagihara Y, Kimura K, Sakai T, Azuma Y, Fujii N, Ohno S

机构信息

Department of Preventive Dentistry, Higashi Nippon Gakuen University, Hokkaido, Japan.

出版信息

J Wildl Dis. 1994 Jul;30(3):439-44. doi: 10.7589/0090-3558-30.3.439.

DOI:10.7589/0090-3558-30.3.439

PMID:7933292

Abstract

Lyme disease spirochetes were demonstrated in a wild female fox (Vulpes vulpes schrencki) and in Ixodes persulcatus ticks collected from the fox on Sapporo, Hokkaido, Japan. Spirochetes were detected in I. persulcatus, as well as skin lesions, brain, heart, kidney, and liver of the fox. Five of seven isolates reacted with a monoclonal antibody against Borrelia afzelii specific Osp B. Deoxyribonucleic acid (DNA) relatedness of a brain isolate was 89% to B. afzelii, and ranged from 50 to 67% to three other species. Immunoglobulin G antibodies to B. afzelii, B. garinii and B. burgdorferi sensulato, when tested in an enzyme-linked immunosorbent assay (ELISA), were negative in the fox. There were no antibodies against seven serovars of Leptospira interrogans.

摘要

在日本北海道札幌市的一只野生雌性赤狐（Vulpes vulpes schrencki）以及从该狐狸身上采集的全沟硬蜱（Ixodes persulcatus）中发现了莱姆病螺旋体。在全沟硬蜱以及狐狸的皮肤病变部位、脑、心脏、肾脏和肝脏中检测到了螺旋体。七株分离株中有五株与抗阿氏疏螺旋体（Borrelia afzelii）特异性外膜蛋白B（Osp B）的单克隆抗体发生反应。一株从脑部分离出的螺旋体的脱氧核糖核酸（DNA）与阿氏疏螺旋体的相关性为89%，与其他三个物种的相关性在50%至67%之间。通过酶联免疫吸附测定（ELISA）检测，该狐狸针对阿氏疏螺旋体、伽氏疏螺旋体（B. garinii）和狭义伯氏疏螺旋体（B. burgdorferi sensulato）的免疫球蛋白G抗体均为阴性。未检测到针对问号钩端螺旋体（Leptospira interrogans）七个血清型的抗体。