Setoyama Y, Imai J, Ishikawa T, Sakaida H, Takaori-Kondo A, Kawamata S, Uchiyama T
Institute for Virus Research, Kyoto University.
Rinsho Byori. 1994 Aug;42(8):834-42.
We measured the serum concentrations of the soluble form of interleukin-2 receptor alpha chain (sIL-2R alpha) in 25 patients with non-Hodgkin lymphoma (NHL) and 1 patient with virus-associated hemophagocytic syndrome (VHAS) by an enzyme-linked immunosorbent assay (ELISA) using two anti-IL-2R alpha monoclonal antibodies which recognize different epitopes. The sIL-2R alpha levels were markedly higher (n = 12, range = 469.2-11020 U/ml, mean +/- SD = 6153.0 +/- 1687.2 U/ml) in the sera of patients with NHL than in healthy individuals (n = 46, range = 290.1-849.3 U/ml, mean +/- SD = 459.8 +/- 126.9 U/ml, p < 0.01). The serum sIL-2R alpha levels in the NHL patients were closely associated with the stage of disease. The mean value of the serum sIL-2R alpha in each stage of NHL was as follows: stage I (469.2 U/ml, n = 1), stage II (4879.0 U/ml, n = 3), stage III (7364.8 U/ml, n = 8), stage IV (13796.2 U/ml, n = 10). However, there was no clear correlation between the elevation of serum sIL-2R alpha level and the pathological (LSG) classification of NHL. The serum sIL-2R alpha levels serially measured during the clinical course of 2 NHL patients were closely associated with the severity and the progression of the disease. Thus, the measurement of serum sIL-2R alpha levels is very useful not only for the diagnosis but also to monitor the clinical course of NHL.
我们使用两种识别不同表位的抗白细胞介素-2受体α链（IL-2Rα）单克隆抗体，通过酶联免疫吸附测定（ELISA）法，检测了25例非霍奇金淋巴瘤（NHL）患者和1例病毒相关性噬血细胞综合征（VHAS）患者血清中可溶性白细胞介素-2受体α链（sIL-2Rα）的浓度。NHL患者血清中的sIL-2Rα水平显著高于健康个体（n = 46，范围 = 290.1 - 849.3 U/ml，均值±标准差 = 459.8 ± 126.9 U/ml，p < 0.01）（n = 12，范围 = 469.2 - 11020 U/ml，均值±标准差 = 6153.0 ± 1687.2 U/ml）。NHL患者血清sIL-2Rα水平与疾病分期密切相关。NHL各期血清sIL-2Rα的均值如下：I期（469.2 U/ml，n = 1），II期（4879.0 U/ml，n = 3），III期（7364.8 U/ml，n = 8），IV期（13796.2 U/ml，n = 10）。然而，血清sIL-2Rα水平升高与NHL的病理（LSG）分类之间没有明显的相关性。在2例NHL患者临床病程中连续检测的血清sIL-2Rα水平与疾病的严重程度和进展密切相关。因此，检测血清sIL-2Rα水平不仅对NHL的诊断非常有用，而且对监测其临床病程也很有用。
