Dalessandri K M, Eisele P, Wong T S, Parker J, Rucker R B
Department of Surgery (112), Palo Alto VA Hospital, CA 94304.
Mech Ageing Dev. 1994 May;74(1-2):117-20. doi: 10.1016/0047-6374(94)90103-1.
The Watanabe hereditary hyperlipidemic (WHHL) rabbit is an animal model that resembles humans with familial hyperlipidemia. In the thoracic aortae, there is also morphologic evidence of marked destruction of medial lamellar elastin fibers. Herein is provided the chemical evidence of elastolytic degradation. The levels of lysyl oxidase (L.Ox.), soluble elastin (SE) and insoluble elastin (IE) were estimated in thoracic aortae samples from New Zealand white (NZW) and WHHL rabbits at 6 months of age or 5 WHHL rabbits at 2.5 years of age. Enzyme-linked immunosorption assays (ELISA) were used in the L.Ox. and SE measurements. IE was measured following alkali extraction of aortae. There was a decrease in IE in thoracic aortae from Watanabe rabbits compared to NZW controls at 6 months of age (P < 0.1), and a further loss of IE in aortae from 2.5-year-old WHHL rabbits relative to the values at 6 months (P < 0.05). Average values for IE were: 130 mg/g for 6-month-old NZW, 100 mg/g for 6-month-old WHHL, and 60 mg/g for 2.5-year-old WHHL rabbits. Moreover, SE was only observed in aorta extracts from the older WHHL rabbits, a sign of elastolytic damage. There was also a five- to sixfold decrease in L.Ox. in the older vs. younger rabbits.
渡边遗传性高脂血症（WHHL）兔是一种类似于人类家族性高脂血症的动物模型。在胸主动脉中，也有中层板层弹性蛋白纤维明显破坏的形态学证据。本文提供了弹性蛋白降解的化学证据。对6月龄新西兰白兔（NZW）和WHHL兔的胸主动脉样本，或5只2.5岁的WHHL兔的胸主动脉样本，测定了赖氨酰氧化酶（L.Ox.）、可溶性弹性蛋白（SE）和不溶性弹性蛋白（IE）的水平。采用酶联免疫吸附测定法（ELISA）测量L.Ox.和SE。在对主动脉进行碱提取后测量IE。与6月龄的NZW对照相比，渡边兔胸主动脉中的IE有所下降（P<0.1），并且相对于6月龄时的值，2.5岁WHHL兔主动脉中的IE进一步减少（P<0.05）。IE的平均值分别为：6月龄NZW兔为130 mg/g，6月龄WHHL兔为100 mg/g，2.5岁WHHL兔为60 mg/g。此外，仅在较年长的WHHL兔的主动脉提取物中观察到SE，这是弹性蛋白降解损伤的一个迹象。与较年轻的兔子相比，较年长兔子的L.Ox.也下降了五到六倍。
