125I-因子XI在遗传性高脂血症兔（WHHL兔）动脉粥样硬化斑块中的蓄积。

Accumulation of 125I-factor XI in atheroma of rabbit with hereditary hyperlipidemia (WHHL-rabbit).

作者信息

Komiyama Y, Masuda M, Murakami T, Nishikado H, Egawa H, Nishimura T, Morii S, Murata K

机构信息

Department of Clinico-laboratory Medicine, Kansai Medical University, Osaka, Japan.

出版信息

Thromb Res. 1989 Oct 1;56(1):49-58. doi: 10.1016/0049-3848(89)90007-8.

DOI:10.1016/0049-3848(89)90007-8

PMID:2595673

Abstract

We have studied the turnover and accumulation of rabbit factor XI (F.XI) in atherosclerotic lesion in Watanabe-hereditable hyperlipidemic rabbit (WHHL rabbit) to reveal the participation of blood coagulation in atherosclerotic lesion. Rabbit F.XI was iodinated and administered intravenously to WHHL rabbits and Japanese white rabbits. The turnover of 125I-rabbit F.XI was significantly faster in WHHL rabbits (T1/2 = 2.84 +/- 0.44 days) than in normal rabbits (T1/2 = 4.44 +/- 0.42 days). The thoracic aorta of WHHL rabbit was strongly labelled with 125I-rabbit F.XI, in sections obtained after 5 days by en-face autoradiography, whereas no radioactivity was detected in normal aorta. By an immunohistochemical study of WHHL rabbit aorta, we confirmed that many F.XI- and fibrin-related compounds existed in the atheroma, whereas albumin did not in these area. These results suggest that the activation of F.XI proceeds on the atherosclerotic lesions of WHHL rabbits.

摘要

我们研究了渡边遗传性高脂血症兔（WHHL兔）动脉粥样硬化病变中兔凝血因子XI（F.XI）的周转和蓄积情况，以揭示血液凝固在动脉粥样硬化病变中的作用。将兔F.XI碘化后静脉注射给WHHL兔和日本白兔。125I-兔F.XI在WHHL兔中的周转速度（T1/2 = 2.84 +/- 0.44天）明显快于正常兔（T1/2 = 4.44 +/- 0.42天）。通过正面放射自显影在5天后获得的切片中，WHHL兔的胸主动脉被125I-兔F.XI强烈标记，而在正常主动脉中未检测到放射性。通过对WHHL兔主动脉的免疫组织化学研究，我们证实动脉粥样瘤中存在许多与F.XI和纤维蛋白相关的化合物，而这些区域中不存在白蛋白。这些结果表明，F.XI的激活在WHHL兔的动脉粥样硬化病变上进行。