Tuomisto L, Yamatodani A, Jolkkonen J, Sainio E L, Airaksinen M M
Department of Pharmacology and Toxicology, University of Kuopio, Finland.
Methods Find Exp Clin Pharmacol. 1994 Jun;16(5):355-9.
Alpha-fluoromethylhistidine (FMH), a histamine synthesis inhibitor, was infused into the lateral cerebral ventricle of male Long-Evans rats for 7 days at a dose of 60 mcg/day. During this period animals were housed in metabolic cages; water and food consumption were measured and urine samples were collected. FMH-treated rats ate significantly more than controls and had a significantly greater weight increase. Concomitantly, sodium and potassium excretion increased. On the seventh day, rats were injected i.p. with 6.67 ml/kg of either 5.8% NaCl or physiological saline. Animals were decapitated 1 h after injection and plasma vasopressin, corticosterone and posterior pituitary vasopressin levels were determined by radioimmunoassay. NaCl loading significantly increased plasma vasopressin in control rats but not in rats pretreated with FMH. FMH alone had no effect. There were no significant changes in pituitary vasopressin or plasma corticosterone. These results clearly suggest an inhibitory role for the histaminergic system in the regulation of food intake. They also agree with, although not proving, the stimulatory control of vasopressin release by the histaminergic system in rat brain.
α-氟甲基组氨酸（FMH）是一种组胺合成抑制剂，以每天60微克的剂量注入雄性Long-Evans大鼠的侧脑室，持续7天。在此期间，动物饲养在代谢笼中；测量水和食物的消耗量，并收集尿液样本。经FMH处理的大鼠比对照组吃得明显更多，体重增加也明显更大。同时，钠和钾的排泄量增加。在第7天，给大鼠腹腔注射6.67毫升/千克的5.8%氯化钠或生理盐水。注射后1小时将动物断头，通过放射免疫测定法测定血浆血管加压素、皮质酮和垂体后叶血管加压素水平。氯化钠负荷使对照大鼠的血浆血管加压素显著增加，但对用FMH预处理的大鼠则无此作用。单独使用FMH没有效果。垂体血管加压素或血浆皮质酮没有显著变化。这些结果清楚地表明组胺能系统在食物摄入调节中具有抑制作用。它们也与组胺能系统对大鼠脑中血管加压素释放的刺激控制作用相符，尽管并未证实这一点。
