Chorvatovicová D, Bauer V
Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava.
Mutagenesis. 1994 May;9(3):241-4. doi: 10.1093/mutage/9.3.241.
The potential antimutagenic effect of stobadine dipalmitate (STB) on the frequency of micronuclei in reticulocytes of peripheral blood in female ICR mice was studied. The cyclophosphamide model was used to verify this effect. Stobadine dipalmitate was administered orally in three concentrations: STB I, 7.07; STB II, 23.6; STB III, 70.07 mg/kg body wt 2 h prior to or 4 h after (STB II only) cyclophosphamide administration (intraperitoneally, twice 80 mg/kg body wt with a 24 h interval). The method designed by Hayashi et al. [(1990) Mutat. Res., 245, 245-249] was used to prepare and to stain the slides. The results of the experiment show that pretreatment with stobadine 2 h prior to cyclophosphamide administration significantly decreased its mutagenic effect, as manifested by the reduced frequency of micronucleated reticulocytes. This protective effect of stobadine was concentration-dependent with the highest concentration of stobadine inducing the most pronounced decrease of micronuclei. Analysis and identification of the exact mechanism of the protective effects of stobadine is the aim of our further studies.
研究了二棕榈酸司他丁（STB）对雌性ICR小鼠外周血网织红细胞微核频率的潜在抗诱变作用。采用环磷酰胺模型验证该作用。在腹腔注射环磷酰胺（两次，每次80mg/kg体重，间隔24小时）前2小时或（仅STB II）后4小时，以三种浓度口服二棕榈酸司他丁：STB I，7.07；STB II，23.6；STB III，70.07mg/kg体重。采用Hayashi等人[(1990) Mutat. Res., 245, 245 - 249]设计的方法制备和染色玻片。实验结果表明，在环磷酰胺给药前2小时用司他丁预处理可显著降低其诱变作用，表现为微核化网织红细胞频率降低。司他丁的这种保护作用呈浓度依赖性，司他丁浓度最高时微核减少最为明显。分析和确定司他丁保护作用的确切机制是我们进一步研究的目标。
