Shi K, Mizuno A, Sano T, Ishida K, Shima K
Department of Laboratory Medicine and Pathology, School of Medicine, University of Tokushima, Japan.
Metabolism. 1994 Oct;43(10):1214-20. doi: 10.1016/0026-0495(94)90213-5.
The incidence of non-insulin-dependent diabetes mellitus in a model rat (Otsuka-Long-Evans-Tokushima Fatty [OLETF]) has been shown to be much higher in males than in females. To evaluate the role of sex hormones in the development of diabetes in this model, we performed biochemical and morphological studies on the effects of castration and sex hormones on the development of non-insulin-dependent diabetes mellitus in these rats. The rats were randomly assigned to six groups of 10 rats each, three groups of males and three of females. Two of the male groups and two of the female groups were castrated at 5 weeks of age, and the third male and female groups received sham operations. From 9 to 30 weeks of age, one group of castrated males received testosterone enanthate (1 mg-wk-1) and one group of castrated females received estradiol valerate (1 mg.wk-1). The other castrated groups received an equal volume of vehicle only. At 30 weeks of age, the cumulative incidences of diabetes mellitus in the sham-operated male and female rats were 100% and 0%, respectively. Orchiectomy reduced the incidence of diabetes to 20%, whereas ovariectomy increased it to 30%. Administration of sex hormones restored the incidence to 89% in males and 0% in females. In vivo insulin-stimulated glucose uptake as measured with a euglycemic clamp was reduced in sham-operated males, castrated males with hormone replacement (HR), and castrated females without HR as compared with sham-operated females and castrated females with HR.(ABSTRACT TRUNCATED AT 250 WORDS)
在一种模型大鼠（大冢-长-艾氏-德岛肥胖大鼠[OLETF]）中，非胰岛素依赖型糖尿病的发病率在雄性中比在雌性中高得多。为了评估性激素在该模型糖尿病发生发展中的作用，我们对去势和性激素对这些大鼠非胰岛素依赖型糖尿病发生发展的影响进行了生化和形态学研究。将大鼠随机分为六组，每组10只，雄性三组，雌性三组。其中两组雄性和两组雌性在5周龄时进行去势，第三组雄性和雌性进行假手术。从9周龄到30周龄，一组去势雄性大鼠接受庚酸睾酮（1毫克/周），一组去势雌性大鼠接受戊酸雌二醇（1毫克/周）。其他去势组仅接受等量的赋形剂。在30周龄时，假手术的雄性和雌性大鼠中糖尿病的累积发病率分别为100%和0%。睾丸切除术将糖尿病发病率降至20%，而卵巢切除术则将其增至30%。给予性激素后，雄性发病率恢复到89%，雌性恢复到0%。与假手术雌性大鼠和接受激素替代（HR）的去势雌性大鼠相比，假手术雄性大鼠、接受HR的去势雄性大鼠和未接受HR的去势雌性大鼠经正常血糖钳夹测量的体内胰岛素刺激的葡萄糖摄取减少。（摘要截断于250字）
