Saito S, Nakano Y, Kushida K, Shirai M, Harada K, Nakano M
Department of Microbiology, Jichi Medical School, Tochigi, Japan.
Microbiol Immunol. 1994;38(5):389-92. doi: 10.1111/j.1348-0421.1994.tb01796.x.
Monoclonal antibodies (MAbs) against the microcystin-leucine-arginine variant (MCYST-LR), a cyclic peptide toxin of the freshwater cyanobacterium Microcystis aeruginosa, were prepared from cloned hybridoma cell lines. The specificity of the MAbs and their ability to neutralize the toxin were investigated by an indirect enzyme-linked immunosorbent assay (ELISA) and by a neutralizing test in mice, respectively. All MAbs reacted with MCYST-LR and also with the microcystin-arginine-arginine variant (MCYST-RR), 3,7-didesmethylmicrocystin (MCYST-3, 7-dDMLR) and 7-desmethylmicrocystin (MCYST-7-DMLR). Furthermore, the antibodies reacted with cell-extracts of toxic and non-toxic M. aeruginosa strains. The MAbs can apparently recognize the common configuration, but not the variant-specific structure, in the microcystin molecules. The non-toxic strains apparently contain some substance(s) related antigenically to microcystin. The in vivo toxin-neutralizing ability of MAbs was minimal.
针对淡水蓝藻铜绿微囊藻的环状肽毒素微囊藻毒素 - 亮氨酸 - 精氨酸变体（MCYST - LR）的单克隆抗体（MAb）是从克隆的杂交瘤细胞系制备的。分别通过间接酶联免疫吸附测定（ELISA）和小鼠中和试验研究了单克隆抗体的特异性及其中和毒素的能力。所有单克隆抗体均与MCYST - LR反应，也与微囊藻毒素 - 精氨酸 - 精氨酸变体（MCYST - RR）、3,7 - 去甲基微囊藻毒素（MCYST - 3,7 - dDMLR）和7 - 去甲基微囊藻毒素（MCYST - 7 - DMLR）反应。此外，这些抗体还与有毒和无毒铜绿微囊藻菌株的细胞提取物反应。单克隆抗体显然可以识别微囊藻毒素分子中的共同构型，但不能识别变体特异性结构。无毒菌株显然含有一些与微囊藻毒素抗原相关的物质。单克隆抗体的体内毒素中和能力极小。
