[通过苯乙酸生成量定量测定青霉素酰化酶活性的方法]

[Method for the quantitative determination of penicillin acylase activity by the formation of phenylacetic acid].

作者信息

Shimaniuk N Ia, Mishan'kin B N

出版信息

Antibiotiki. 1976 Aug;21(8):694-7.

Abstract

The quantitative method for determination of penicillinacylase activity is described. The method is based on detection of phenylacetic acid (PAA) formed during hydrolysis of benzylpenicillin. PAA is extracted with toluol and nitrated with potassium nitrate solution in concentrated sulphuric acid followed by reduction of nitrophenylacetic acid into aminophenylacetic acid with zinc powder. Aminophenylacetic acid interacts with p-dimethylaminobenzaldehyde in acid medium forming a coloured compound (Schiff base). The optic density of the latter was determined with spectrophotometer SF-16 at 430 nm. The method was used for determination of acylase in Yersinia species and some other bacteria.

摘要

描述了一种测定青霉素酰化酶活性的定量方法。该方法基于检测苄青霉素水解过程中形成的苯乙酸（PAA）。用甲苯萃取PAA，然后在浓硫酸中用硝酸钾溶液硝化，接着用锌粉将硝基苯乙酸还原为氨基苯乙酸。氨基苯乙酸在酸性介质中与对二甲基氨基苯甲醛相互作用形成有色化合物（席夫碱）。用SF - 16型分光光度计在430nm处测定后者的光密度。该方法用于测定耶尔森氏菌属和其他一些细菌中的酰化酶。

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引用本文的文献

1

Penicillin acylases. An update.青霉素酰化酶。最新进展。

Appl Biochem Biotechnol. 1984 Oct-Dec;9(5-6):537-54. doi: 10.1007/BF02798404.

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