Schneider H C, Berthold J, Bauer M F, Dietmeier K, Guiard B, Brunner M, Neupert W
Institut für Physiologische Chemie der Universität München, Germany.
Nature. 1994 Oct 27;371(6500):768-74. doi: 10.1038/371768a0.
Protein translocation into mitochondria requires the mitochondrial protein Hsp70. This molecular chaperone of the mitochondrial matrix is recruited to the protein import machinery by MIM44, a component associated with the inner membrane of the mitochondria. Formation of the mt-Hsp70/MIM44 complex is regulated by ATP. MIM44 and mt-Hsp 70 interact in a sequential manner with incoming segments of unfolded preproteins and thereby facilitate stepwise vectorial translocation of proteins across the mitochondrial membranes. The complex appears to act as a molecular ratchet which is energetically driven by the hydrolysis of ATP.
蛋白质转运到线粒体中需要线粒体蛋白Hsp70。线粒体基质的这种分子伴侣由MIM44招募到蛋白质导入机制中，MIM44是一种与线粒体内膜相关的成分。mt-Hsp70/MIM44复合物的形成受ATP调节。MIM44和mt-Hsp 70与未折叠前体蛋白的进入片段依次相互作用，从而促进蛋白质在线粒体内膜上的逐步向量转运。该复合物似乎起着分子棘轮的作用，由ATP水解提供能量驱动。
