González-Darder J M, Segura-Pastor D
Department of Neurosurgery, Faculty of Medicine, University of Cádiz, Spain.
Neurol Res. 1994 Jun;16(3):187-90. doi: 10.1080/01616412.1994.11740224.
Laser Doppler flowmetry was used as a method of measurement of skin blood flow perfusion in an experimental rat model of neuropathic pain. The common sciatic nerve was microsurgically approached and four ligatures were loosely tied around the nerve (sciatic ligature group). Contralateral nerves were approached but not injured (sham procedure). All paws were studied before any surgical procedure (controls) and immediately, 24 h, 11 and 25 days after surgery. In each experiment, the skin perfusion was measured in the plantar surface of the hind paw, both in baseline conditions and after a heating test, where skin temperature around flowmeter probe reached 42 degrees C. The heating test produced an increase in skin perfusion that ranged between 70% and 80% in sham and normal paws. Nerve ligation induced no immediate changes in skin perfusion with a normal 65% increase after heating. However, 24 h later, baseline skin perfusion was significantly higher than preoperatively with smaller response to heating. This situation changed at 11 and 25 days, with baseline values significantly smaller than before surgery and responses to heating significantly higher. Nerve section in these previously ligated sciatic nerves produces a significant increase in skin perfusion. Our results suggest that vasomotor changes in the model of sciatic ligature are presumably related to a denervation hypersensitivity of skin blood vessels to vasoactive agents.
激光多普勒血流仪被用作在神经性疼痛实验大鼠模型中测量皮肤血流灌注的一种方法。通过显微手术暴露坐骨神经，并在神经周围松散地系上四个结扎线（坐骨神经结扎组）。对侧神经也进行暴露但不损伤（假手术）。在任何手术操作前（对照组）以及手术后立即、24小时、11天和25天对所有爪子进行研究。在每个实验中，在基础状态下以及加热测试后测量后爪足底表面的皮肤灌注，加热测试时流量计探头周围的皮肤温度达到42摄氏度。加热测试使假手术组和正常爪子的皮肤灌注增加70%至80%。神经结扎后皮肤灌注没有立即变化，加热后正常增加65%。然而，24小时后，基础皮肤灌注显著高于术前，对加热的反应较小。这种情况在11天和25天时发生变化，基础值显著小于手术前，对加热的反应显著更高。对这些先前结扎的坐骨神经进行切断会使皮肤灌注显著增加。我们的结果表明，坐骨神经结扎模型中的血管舒缩变化可能与皮肤血管对血管活性物质的去神经超敏反应有关。
