González-Darder J M, Segura-Pastor D
Department of Neurosurgery, Faculty of Medicine, University of Cádiz, Spain.
Neurol Res. 1994 Jun;16(3):191-3. doi: 10.1080/01616412.1994.11740225.
Laser Doppler flowmetry was used as a method of measurement of skin blood flow perfusion in an experimental rat model of peripheral nerve section. The common sciatic nerve was microsurgically approached and cleanly severed (sciatic transection group). Contralateral nerves were approached but not injured (sham procedure). All paws were studied before any surgical procedure (controls) and immediately, 24 hours, 11 and 25 days after surgery. In each experiment, the skin perfusion was measured in the plantar surface of the hind paw, both in baseline conditions and after a heating test, where skin temperature around flowmeter probe reached 42 degrees C. Sham operated paws showed no differences from control unoperated paws. The heating test produced an increase in skin perfusion that ranged between 70% and 80% in sham and normal paws. Sciatic nerve section produces an immediate increase of 36.7% in blood flow with an additional increase of 27.5% after heating. In the following studies, on days 1, 11 and 25 baseline skin perfusion showed no changes in relation to preoperative values and heating induced only a small increase in perfusion.
激光多普勒血流仪被用作测量周围神经切断实验大鼠模型中皮肤血流灌注的一种方法。通过显微外科手术暴露并干净地切断坐骨神经（坐骨神经切断组）。对侧神经也进行暴露但不损伤（假手术组）。在任何手术操作前（对照组）以及手术后立即、24小时、11天和25天对所有爪子进行研究。在每个实验中，在后爪的足底表面测量皮肤灌注，包括基线条件下以及加热测试后，加热测试时流量计探头周围的皮肤温度达到42摄氏度。假手术组的爪子与未手术的对照爪子没有差异。加热测试使假手术组和正常爪子的皮肤灌注增加了70%至80%。坐骨神经切断后血流立即增加36.7%，加热后又额外增加27.5%。在接下来的研究中，在第1天、11天和25天，基线皮肤灌注与术前值相比没有变化，加热仅引起灌注的小幅增加。
