成年大鼠坐骨神经睫状神经营养因子的纯化

Purification of adult rat sciatic nerve ciliary neuronotrophic factor.

作者信息

Manthorpe M, Skaper S D, Williams L R, Varon S

出版信息

Brain Res. 1986 Mar 5;367(1-2):282-6. doi: 10.1016/0006-8993(86)91603-3.

Abstract

The ciliary neuronotrophic factor (CNTF), a protein required for the survival of cultured avian embryonic parasympathetic ciliary ganglionic neurons, was recently purified from extracts of selected chick intraocular tissues. Here we report the purification of a mammalian CNTF activity from extracts of adult rat sciatic nerve using a fractionation procedure similar to that employed for isolating chick eye CNTF. About 2 micrograms of CNTF protein can be obtained from each 1.5 g batch of nerve tissue. Like the chick CNTF, the mammalian factor displays trophic activity for dorsal root and sympathetic as well as ciliary ganglionic neurons. The nerve CNTF activity differs from its chick counterpart in molecular weight and chromatographic behavior on ion-exchange columns. Unlike purified nerve growth factor (NGF), nerve CNTF activity is insensitive to anti-NGF antibodies and is unable to support the survival of 8-day chick embryo dorsal root ganglion neurons.

摘要

睫状神经营养因子（CNTF）是培养的禽胚胎副交感睫状神经节神经元存活所必需的一种蛋白质，最近已从选定的鸡眼内组织提取物中纯化出来。在此，我们报告使用与分离鸡眼CNTF所用方法类似的分级分离程序，从成年大鼠坐骨神经提取物中纯化出一种哺乳动物CNTF活性物质。每1.5克神经组织批次可获得约2微克的CNTF蛋白。与鸡CNTF一样，这种哺乳动物因子对背根神经节、交感神经节以及睫状神经节神经元均表现出营养活性。神经CNTF活性物质在分子量和离子交换柱上的色谱行为方面与其鸡源对应物不同。与纯化的神经生长因子（NGF）不同，神经CNTF活性物质对抗NGF抗体不敏感，且无法支持8日龄鸡胚背根神经节神经元的存活。